Journal of Experimental Pharmacology (Oct 2022)
Estimation of Platelet Count and Bleeding Time of Mice Treated with Musa paradisiaca var. sapientum (L.) Kuntze Extract
Abstract
Hendrik Setia Budi,1 Doaa Elsayed Ramadan,2,3 Silvia Anitasari,4,5 Elza Widya Pangestika6 1Department of Oral Biology, Dental Pharmacology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; 2Doctoral Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; 3Directorate of Damietta Health Affairs, Ministry of Health and Population, Cairo, Egypt; 4Department of Dental Material and Devices, Dentistry Program, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia; 5School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; 6Undergraduate Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaCorrespondence: Hendrik Setia Budi, Department of Oral Biology, Dental Pharmacology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia, Tel/Fax +62315020255, Email [email protected]: The aim of this study was to estimate the platelet count and bleeding time on peripheral blood smear of mice tail wound using Musa paradisiaca var. sapientum (L.) Kuntze (ambonese banana stem extract).Design: Randomized post-test-only control group design.Materials and Methods: Twenty-four male mice (Mus Musculus) were randomly divided into 4 groups. A negative control group was treated with carboxymethyl cellulose (CMC), a positive control group (K+) treated aspirin 100 mg/kg body weight, group P1 treated with aspirin 100 mg/kg body weight and tranexamic acid 50 mg/kg body weight, and group P2 treated with 30% of ambonese banana stem extract (ABSE). The mean and standard deviation data of platelet counts and bleeding time were analyzed by one-way ANOVA statistical software.Results and Discussion: Tranexamic acid had no significant effect on platelets count compared to CMC group (p = 0.871), but administration of aspirin resulted in low platelets count significantly (p = 0.003). The platelet counts of ABSE and CMC groups were not significant different (p = 0.937). Aspirin has significantly shown prolonged bleeding time than CMC, tranexamic acid, and ABSE groups. However, there was no difference between the tranexamic acid and ABSE groups (p=0.934). The bleeding time of tranexamic acid and ABSE groups was similar, although the platelet count in the ABSE group was lower than in the CMC group.Conclusion: This study proved that ambonese banana stem extract has a potency to shorten the bleeding time in mice tail wound without interfering to platelet count.Graphical Abstract: Keywords: haemorrhage, health risk, herbal medicine, peripheral blood smears, wound
