Acta Societatis Botanicorum Poloniae (Jan 2011)

Potential use of RAPD markers in characteristics of cucumber (Cucumis sativus L.) haploids and double-haploids

  • Katarzyna Niemirowicz-Szczytt,
  • Grażyna Kołakowska,
  • Aleksandra Korzeniewska,
  • Leszek Marzec,
  • Teresa Gałecka,
  • Urszula Piskurewicz,
  • Joanna Sztangret-Wilniewska,
  • Mieczysław Śmiech

DOI
https://doi.org/10.5586/asbp.2008.004
Journal volume & issue
Vol. 77, no. 1
pp. 29 – 34

Abstract

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A study was designed to obtain hapolid and double haploid (DH) plants from cucumber cultivars tolerant to Pseudoperonospora cubensis. The main goal was to identify RAPD molecular markers associated with downy mildew resistance. On average, 20% of embryos generated in two experiments, were converted to haploid plants. RAPD markers that differentiated susceptible and resistant H and DH plants were identified. Somaclonal variation in DH lines was seldom detected.

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