Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms

Analú Barros de Oliveira; Túlio Morandin Ferrisse; Gabriela Gomes França; Sarah Raquel de Annunzio; Willian Kopp; Carla Raquel Fontana; Fernanda Lourenção Brighenti

doi:10.3390/pathogens12020155

Pathogens (Jan 2023)

Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms

Analú Barros de Oliveira,
Túlio Morandin Ferrisse,
Gabriela Gomes França,
Sarah Raquel de Annunzio,
Willian Kopp,
Carla Raquel Fontana,
Fernanda Lourenção Brighenti

Affiliations

Analú Barros de Oliveira: Department of Morphology and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara 14801-903, SP, Brazil
Túlio Morandin Ferrisse: Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara 14801-903, SP, Brazil
Gabriela Gomes França: Department of Morphology and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara 14801-903, SP, Brazil
Sarah Raquel de Annunzio: Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara 14801-903, SP, Brazil
Willian Kopp: Department of Morphology and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara 14801-903, SP, Brazil
Carla Raquel Fontana: Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, SP, Brazil
Fernanda Lourenção Brighenti: Department of Morphology and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara 14801-903, SP, Brazil

DOI: https://doi.org/10.3390/pathogens12020155
Journal volume & issue: Vol. 12, no. 2
p. 155

Abstract

Read online

The synergic effect of Streptococcus mutans and Candida albicans increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two different Brazilian green propolis (BGP-AF and BGP-AG) as PS for aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts and their fractions and controls. Such extracts showed the best results and were evaluated in dual-species biofilms. Photodegradation, reactive oxygen species (ROS), cytotoxicity, and color stability assays were also investigated. Reductions higher than 3 log10 CFU/mL (p p S. mutans and C. albicans regrowth after 24 h of treatment (p p > 0.05). BGP-AF-mediated aPDT showed 72.41% of oral keratinocyte viability (p S. mutans and C. albicans. Specifically, BGP-AF may represent a promising PS for dental applications.

Published in Pathogens

ISSN: 2076-0817 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/pathogens

About the journal

Abstract

Keywords