Data in Brief (Sep 2016)

Data on the identity and myristoylation state of recombinant, purified hippocalcin

  • Anuradha Krishnan,
  • Jeffrey Viviano,
  • Yaroslav Morozov,
  • Venkat Venkataraman

Journal volume & issue
Vol. 8
pp. 78 – 81

Abstract

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In this data article we report on the purity and post translation modification of bacterially expressed and purified recombinant hippocalcin (HPCA): a member of the neuronal calcium sensor protein family, whose functions are regulated by calcium. MALDI-TOF in source decay (ISD) analysis was used to identify both the myristoylated or non-myristoylated forms of the protein. MALDI-TOF ISD data on the identity of the protein, amino acid sequence and myristoylation efficiency are provided. This data relates to the article “Single-Column Purification of the Tag-free, Recombinant Form of the Neuronal Calcium Sensor Protein, Hippocalcin Expressed in Eschericia coli” [1]. Keywords: Hippocalcin, Calcium, NCS proteins, MALDI-TOF, ISD sequencing, Myristoylation