Jurnal Vektor Penyakit (Nov 2018)

Efektivitas Biolarvasida Ekstrak Fraksi Polar Daun Citrus hystrix dan Citrus aurantifolia terhadap Culex quinquefasciatus

  • Arif Nur Muhammad Ansori,
  • Hebert Adrianto,
  • Hamidah Hamidah

DOI
https://doi.org/10.22435/vektorp.v12i1.261
Journal volume & issue
Vol. 12, no. 1
pp. 33 – 38

Abstract

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Abstract The development of resistance to chemical insecticides against mosquitoes has been considered as a setback in vector control. This study was aimed to identify Lethal Concentration 90% (LC90) of polar fraction extract from Citrus hystrix and Citrus aurantifolia leaves as biolarvicide against mosquito larvae after 24 hours of exposure. This study was an experimental research based on a Completely Randomized Design (CRD). The polar fraction extract of C. hystrix and C. aurantifolia were tested in concentrations of 0 ppm, 500 ppm, 1375 ppm, 2250 ppm, 3125 ppm, and 4000 ppm against the 3rd instar larvae of Culex quinquefasciatus. Each treatment was done in five independent replications. The numbers of deceased mosquito larvae were determined after 24 hours of treatment and analyzed by probit. The results showed that the polar fraction extract of C. hystrix and C. aurantifolia have bio-larvicidal activity against the 3rd instar larvae of Cx. quinquefasciatus at LC90 of 1,653 ppm and 2,797 ppm, respectively. In conclusion, the polar fraction extract of C. hystrix leaf is more toxic than C. aurantifolia leaf's against Cx. quinquefasciatus larvae. Abstrak Perkembangan resistensi terhadap insektisida kimia di antara spesies nyamuk telah dianggap sebagai kemunduran dalam pengendalian vektor. Penelitian ini bertujuan untuk mengetahui Lethal Concentration 90% (LC90 ) ekstrak fraksi polar daun C. hystrix dan C. aurantifolia sebagai biolarvasida terhadap larva nyamuk setelah paparan 24 jam. Penelitian ini adalah penelitian eksperimental dengan Rancangan Acak Lengkap (RAL). Ekstrak fraksi polar daun C. hystrix dan C. aurantifolia diuji dengan konsentrasi 0 ppm, 500 ppm, 1.375 ppm, 2.250 ppm, 3.125 ppm, dan 4.000 ppm terhadap larva nyamuk Cx. quinquefasciatus instar III. Setiap konsentrasi dilakukan replikasi sebanyak lima kali. Jumlah larva nyamuk yang mati dihitung setelah 24 jam. Setelah itu, data dari larva nyamuk yang mati dianalisis menggunakan probit. Hasil penelitian ini mengungkapkan bahwa ekstrak fraksi polar daun C. hystrix dan C. aurantifolia memiliki aktivitas sebagai biolarvasida terhadap larva nyamuk Cx. quinquefasciatus instar III dengan LC 90= 1.653 ppm dan 2.797 ppm, secara berurutan. Kesimpulan dari penelitian ini adalah ekstrak fraksi polar dari daun C. hystrix lebih toksik dibandingkan dengan C. aurantifolia terhadap larva nyamuk instar III.

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