Integrated 16S rDNA sequencing and metabolomics to explore the intestinal changes in children and rats with dental fluorosis

Guoyu Zhou; Qingyuan Li; Xiangbo Hou; Huiying Wu; Xiaoli Fu; Guoqing Wang; Jun Ma; Xuemin Cheng; Yang Yang; Ruiqin Chen; Zhiyuan Li; Fangfang Yu; Jingyuan Zhu; Yue Ba

Ecotoxicology and Environmental Safety (Feb 2023)

Integrated 16S rDNA sequencing and metabolomics to explore the intestinal changes in children and rats with dental fluorosis

Guoyu Zhou,
Qingyuan Li,
Xiangbo Hou,
Huiying Wu,
Xiaoli Fu,
Guoqing Wang,
Jun Ma,
Xuemin Cheng,
Yang Yang,
Ruiqin Chen,
Zhiyuan Li,
Fangfang Yu,
Jingyuan Zhu,
Yue Ba

Affiliations

Guoyu Zhou: Department of Environment Health, School of Public Health of Zhengzhou University, Zhengzhou, Henan, PR China; Yellow River Institute for Ecological Protection & Regional Coordinated Development, Zhengzhou University, Zhengzhou, Henan, PR China; National Health Commission Key Laboratory of Birth Defects Prevention, Henan Key Laboratory of Population Defects Prevention, Zhengzhou, Henan, PR China
Qingyuan Li: Department of Environment Health, School of Public Health of Zhengzhou University, Zhengzhou, Henan, PR China
Xiangbo Hou: Department of Environment Health, School of Public Health of Zhengzhou University, Zhengzhou, Henan, PR China
Huiying Wu: Department of Environment Health, School of Public Health of Zhengzhou University, Zhengzhou, Henan, PR China
Xiaoli Fu: Department of Environment Health, School of Public Health of Zhengzhou University, Zhengzhou, Henan, PR China
Guoqing Wang: Department of Environment Health, School of Public Health of Zhengzhou University, Zhengzhou, Henan, PR China
Jun Ma: Department of Endemic Disease, Kaifeng Center for Disease Control and Prevention, Kaifeng, Henan, PR China
Xuemin Cheng: Department of Environment Health, School of Public Health of Zhengzhou University, Zhengzhou, Henan, PR China
Yang Yang: Department of Preventive Medicine, School of Public Health, Zhengzhou University, Zhengzhou, Henan, PR China
Ruiqin Chen: Jinshui District Center for Disease Control and Prevention, Zhengzhou, Henan, PR China
Zhiyuan Li: Department of Environment Health, School of Public Health of Zhengzhou University, Zhengzhou, Henan, PR China
Fangfang Yu: Department of Environment Health, School of Public Health of Zhengzhou University, Zhengzhou, Henan, PR China
Jingyuan Zhu: Department of Environment Health, School of Public Health of Zhengzhou University, Zhengzhou, Henan, PR China; Correspondence to: Department of Environment Health, School of Public Health, Zhengzhou University, 100 Science Avenue, Zhengzhou, Henan, PR China.
Yue Ba: Department of Environment Health, School of Public Health of Zhengzhou University, Zhengzhou, Henan, PR China; Yellow River Institute for Ecological Protection & Regional Coordinated Development, Zhengzhou University, Zhengzhou, Henan, PR China; Correspondence to: Department of Environment Health, School of Public Health, Zhengzhou University, 100 Science Avenue, Zhengzhou, Henan, PR China.

Journal volume & issue: Vol. 251
p. 114518

Abstract

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Dental fluorosis (DF) is a widely prevalent disease caused by excessive fluoride with limited awareness of its underlying pathogenesis. Here, a pilot population study was conducted to explore the pathogenesis of DF from the perspective of intestinal microbiome changes, and verified it in animal experiments combining intestinal microbiome and metabolomics. A total of 23 children were recruited in 2017 in China and divided into DF (n = 9) and control (n = 14) groups (DFG and CG, respectively). The SD rat model was established by drinking water containing sodium fluoride (NaF). Gut microbiome profiles of children and rats were analyzed by16S rDNA V3-V4 sequencing, and the intestinal metabolomics analysis of rats was performed by LC-MS methods. The 16 S rDNA sequencing revealed that the gut microbiome composition was significantly perturbed in children in DFG compared to that in CG. Acidobacteria and Thermi were specifically observed in DFG and CG, respectively. Besides, 15 fecal microbiotas were significantly altered at the genus level in DFG. Furthermore, only the expression of annotated genes for pentose and glucuronate interconversion pathway was significant lower in DFG than that in CG (P = 0.04). Notably, in NaF-treated rats, we also observed the changes of some key components of pentose and glucuronate interconversion pathway at the level of microorganisms and metabolites. Our findings suggested that the occurrence of DF is closely related to the alteration of intestinal microorganisms and metabolites annotated in the pentose and glucuronate interconversion pathway.

Published in Ecotoxicology and Environmental Safety

ISSN: 0147-6513 (Print); 1090-2414 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Technology: Environmental technology. Sanitary engineering: Environmental pollution; Geography. Anthropology. Recreation: Environmental sciences
Website: https://www.journals.elsevier.com/ecotoxicology-and-environmental-safety

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