Studies on green synthesized silver nanoparticles using Abelmoschus esculentus (L.) pulp extract having anticancer (in vitro) and antimicrobial applications

Md. Masud Rahaman Mollick; Dipak Rana; Sandeep Kumar Dash; Sourav Chattopadhyay; Biplab Bhowmick; Dipanwita Maity; Dibyendu Mondal; Sutanuka Pattanayak; Somenath Roy; Mukut Chakraborty; Dipankar Chattopadhyay

Arabian Journal of Chemistry (Dec 2019)

Studies on green synthesized silver nanoparticles using Abelmoschus esculentus (L.) pulp extract having anticancer (in vitro) and antimicrobial applications

Md. Masud Rahaman Mollick,
Dipak Rana,
Sandeep Kumar Dash,
Sourav Chattopadhyay,
Biplab Bhowmick,
Dipanwita Maity,
Dibyendu Mondal,
Sutanuka Pattanayak,
Somenath Roy,
Mukut Chakraborty,
Dipankar Chattopadhyay

Affiliations

Md. Masud Rahaman Mollick: Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata 700 009, India
Dipak Rana: Department of Chemical and Biological Engineering, Industrial Membrane Research Institute, University of Ottawa, 161 Louis Pasteur St., Ottawa, ON K1N 6N5, Canada
Sandeep Kumar Dash: Department of Human Physiology with Community Health, Immunology and Microbiology Laboratory, Vidyasagar University, Midnapore 721 102, India
Sourav Chattopadhyay: Department of Human Physiology with Community Health, Immunology and Microbiology Laboratory, Vidyasagar University, Midnapore 721 102, India
Biplab Bhowmick: Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata 700 009, India
Dipanwita Maity: Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata 700 009, India
Dibyendu Mondal: Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata 700 009, India
Sutanuka Pattanayak: Department of Chemistry, West Bengal State University, Barasat, Kolkata 700 126, India
Somenath Roy: Department of Human Physiology with Community Health, Immunology and Microbiology Laboratory, Vidyasagar University, Midnapore 721 102, India
Mukut Chakraborty: Department of Chemistry, West Bengal State University, Barasat, Kolkata 700 126, India; Corresponding authors. Tel.: +91 33 2350 1397/6996/6387/8386; fax: +91 33 2351 9755.
Dipankar Chattopadhyay: Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata 700 009, India; Corresponding authors. Tel.: +91 33 2350 1397/6996/6387/8386; fax: +91 33 2351 9755.

Journal volume & issue: Vol. 12, no. 8
pp. 2572 – 2584

Abstract

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Silver nanoparticles (Ag NPs) were successfully synthesized using AgNO3 via an eco-friendly and simple green route using Abelmoschus esculentus (L.) pulp extract at room temperature. The phytochemicals present in A. esculentus (L.) pulp extract were used both as a reducing and a stabilizing agent for the synthesis of Ag NPs. The stabilization of Ag NPs with phytochemicals was justified using Fourier-transform infrared spectroscopy. The size of the as-synthesized Ag NPs was examined using dynamic light scattering and confirmed by transmission electron microscopy. The crystalline nature of Ag NPs had been identified using X-ray diffraction. The present study demonstrated the efficacy of Ag NPs against Jurkat cells in vitro. Our study also showed that the IC50 dose of Ag NPs leads to the increase in intracellular reactive oxygen species and significantly diminished mitochondrial membrane potential, indicating the effective involvement of apoptosis in cell death. The synthesized Ag NPs also exhibited good antimicrobial activity against different gram class bacteria. Keywords: Silver nanoparticles, Abelmoschus esculentus (L), Anticancer activity (in vitro), Nitric oxide release, Caspase-3 activity, Antimicrobial activity

Published in Arabian Journal of Chemistry

ISSN: 1878-5352 (Print)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry
Website: http://www.journals.elsevier.com/arabian-journal-of-chemistry/

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