Frontiers in Genetics (Jun 2021)
MicroRNA-mRNA Regulatory Networking Fine-Tunes Polyunsaturated Fatty Acid Synthesis and Metabolism in the Inner Mongolia Cashmere Goat
- Yuchun Xie,
- Yuchun Xie,
- Yuchun Xie,
- Yuchun Xie,
- Zhihong Liu,
- Zhihong Liu,
- Zhihong Liu,
- Zhihong Liu,
- Juntao Guo,
- Juntao Guo,
- Juntao Guo,
- Juntao Guo,
- Xin Su,
- Xin Su,
- Xin Su,
- Xin Su,
- Cun Zhao,
- Cun Zhao,
- Cun Zhao,
- Cun Zhao,
- Chongyan Zhang,
- Chongyan Zhang,
- Chongyan Zhang,
- Chongyan Zhang,
- Qing Qin,
- Qing Qin,
- Qing Qin,
- Qing Qin,
- Dongliang Dai,
- Dongliang Dai,
- Dongliang Dai,
- Dongliang Dai,
- Yanhong Zhao,
- Yanhong Zhao,
- Yanhong Zhao,
- Yanhong Zhao,
- Zhiying Wang,
- Zhiying Wang,
- Zhiying Wang,
- Zhiying Wang,
- Ruijun Wang,
- Ruijun Wang,
- Ruijun Wang,
- Ruijun Wang,
- Yanjun Zhang,
- Yanjun Zhang,
- Yanjun Zhang,
- Yanjun Zhang,
- Rui Su,
- Rui Su,
- Rui Su,
- Rui Su,
- Zhixin Wang,
- Zhixin Wang,
- Zhixin Wang,
- Zhixin Wang,
- Jinquan Li,
- Jinquan Li,
- Jinquan Li,
- Jinquan Li
Affiliations
- Yuchun Xie
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Yuchun Xie
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Yuchun Xie
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Yuchun Xie
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Zhihong Liu
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Zhihong Liu
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Zhihong Liu
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Zhihong Liu
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Juntao Guo
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Juntao Guo
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Juntao Guo
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Juntao Guo
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Xin Su
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Xin Su
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Xin Su
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Xin Su
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Cun Zhao
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Cun Zhao
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Cun Zhao
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Cun Zhao
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Chongyan Zhang
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Chongyan Zhang
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Chongyan Zhang
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Chongyan Zhang
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Qing Qin
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Qing Qin
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Qing Qin
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Qing Qin
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Dongliang Dai
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Dongliang Dai
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Dongliang Dai
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Dongliang Dai
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Yanhong Zhao
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Yanhong Zhao
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Yanhong Zhao
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Yanhong Zhao
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Zhiying Wang
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Zhiying Wang
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Zhiying Wang
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Zhiying Wang
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Ruijun Wang
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Ruijun Wang
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Ruijun Wang
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Ruijun Wang
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Yanjun Zhang
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Yanjun Zhang
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Yanjun Zhang
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Yanjun Zhang
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Rui Su
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Rui Su
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Rui Su
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Rui Su
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Zhixin Wang
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Zhixin Wang
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Zhixin Wang
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Zhixin Wang
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- Jinquan Li
- College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
- Jinquan Li
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China
- Jinquan Li
- Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
- Jinquan Li
- Engineering Research Center for Goat Genetics and Breeding, Hohhot, China
- DOI
- https://doi.org/10.3389/fgene.2021.649015
- Journal volume & issue
-
Vol. 12
Abstract
Fatty acid composition is an important aspect of meat quality in ruminants. Improving the beneficial fatty acid level in cashmere goat meat is important to its economic value. To investigate microRNAs (miRNAs) and mRNAs that regulate or coregulate polyunsaturated fatty acid (PUFA) synthesis and metabolism in the Inner Mongolia cashmere goat, we used longissimus dorsi muscle (WLM) and biceps femoris muscle (WBM) for transcript-level sequencing. RT-qPCR was used to evaluate the expression of mRNAs and miRNAs associated with PUFA synthesis and metabolism. The total PUFA content in the WBM was significantly higher than that in the WLM (P < 0.05). Our study is the first to systematically report miRNAs in cashmere goat meat. At the mRNA level, 20,375 genes were identified. ACSL1, CD36 and TECRL were at the center of a gene regulatory network and contributed significantly to the accumulation and metabolic regulation of fatty acids. At the miRNA level, 426 known miRNAs and 30 novel miRNAs were identified. KEGG analysis revealed that the miRNA target genes were involved mainly in the PPAR signaling pathway. The mRNA-miRNA coregulation analysis showed that ACSL1 was negatively targeted by nine miRNAs: chi-miR-10a-5p, chi-miR-10b-5p, chi-miR-130b-5p, chi-miR-15a-5p_R-1, chi-miR-15b-5p, chi-miR-16a-5p, chi-miR-16b-5p, chi-miR-181c-5p_R+1, and chi-miR-26b-5p. Finally, we speculated that the simultaneous silencing of ACSL1 by one or more of these nine miRNAs through PPAR signaling led to low ACSL1 expression in the WLM and, ultimately to high PUFA content in the WBM. Our study helps elucidate the metabolic regulation of fatty acids in Inner Mongolia cashmere goats.
Keywords