Histopathological investigation of the effect of Viola odorata and Pistacia atlantica extract on acute tendonitis in rats

Abstract

Background & Aim: Tendon is the connective tissue between bone and muscle. Because of poor blood flow and the possibility of adhesions during repair, tendon injury management is particularlyimportant.Therefore, finding a quick way to repair tendon tissue is one of the targets that has been pursued over the years. Experimental: In this study, 24 male rats were divided into 4 groups, including first group without any treatment, second group receiving dexamethasone (1mg/kg), third group receiving 250 mg/kg Viola odorata and Pistacia atlantica extract, fourth group receiving 500 mg/kg and Pistacia atlantica and Viola odorata extract. After anesthesia of rat acute tendonitis developed. Al the mentioned groups were injected subcutaneously on bigining day, 7 and 21 days after surgery and histopathological evaluation was performed on day 21. Results: The results showed no statistical significance in the number of fibroblasts between different groups. However, dexamethasone groups and the one receiving 500 mg/kg of extract differed significantly from the control group in terms of inflammation and bleeding. Furthermore, the number of fibrocytes in the groups receiving 500 mg/kg and 250 mg/kg doses of Viola odorata and Pistacia atlantica extracts was significantly different from the control and dexamethasone groups (P <0.05). Recommended applications/industries: According to the results, the amount of collagen and fibrocytes in the group treated with 500 mg/kg had a significant difference, compared to other groups, probably due to the anti-inflammatory, antibacterial and antioxidant properties of Viola odorata and Pistacia atlantica. They can be used in traditional medicine along with other medicines.

Keywords

• acute tendonitis
• histopathology
• pistacia atlantica
• rat