A Comparative Study of Fungal Community Structure, Diversity and Richness between the Soil and the Phyllosphere of Native Grass Species in a Copper Tailings Dam in Shanxi Province, China

Abstract

In the study area, mining processes have led to the accumulation of a large amount of ore sand and a tailings dam was established above this artificial overburden. After a long period of restoration, the area was reclaimed by a variety of native vegetation. This study investigated four of these native grass species, namely, Bothriochloa ischaemum, Imperata cylindrica, Elymus dahuricus and Calamagrostis epigejos, having reestablished themselves after the restoration of a copper tailings dam built in 1969 in Shanxi Province, China. We analyzed the fungal community structure in the soil and the phyllosphere of the four native grass species using high-throughput sequencing. Results showed that the soil of the tailings dam was weakly alkaline and copper (Cu) was the most pervasive element present. Ascomycota were the dominant fungal taxa in the soil and the phyllosphere of all four native grass species, for which total soil nitrogen (N) content was an influencing factor. Basidiomycota was positively correlated to cadmium (Cd), which can additionally be used as an indicator of Cd pollution in copper tailings dams. Among the four native grass species, Nectriaceae was the dominant fungal family found exclusively in B. ischaemum; Meruliaceae and Phaeosphaeriaceae were the dominant fungal families of E. dahuricus; Cordycipitaceae and Sporormiaceae were only found in C. epigejos. However, we found no evidence of a dominant fungal family in I. cylindrica. Furthermore, Erythrobasidiales sp., which had the highest betweenness centrality after network analysis, was identified as the key fungal species in all four native grass species.

Keywords

• copper tailings dam
• native grasses
• phyllosphere fungi
• soil fungal community