Soybean Germination Response to Algae Extract and a Static Magnetic Field Treatment

Abstract

The present study examines the separate and synergistic effects of macroalgal extract and static magnetic field (SMF) on the germination of soybean seeds (Glycine max (L.) Merrill), cv. Abelina, seedling growth, chlorophyll, and carotenoids content in leaves. Algal extract was produced from freshwater green macroalga (Cladophora glomerata) using ultrasound-assisted extraction. The germination tests were conducted in two stages. Firstly, different concentrations of extracts, 20%, 40%, 60%, 80%, and 100%, were applied to a paper substrate. The best results (taking into account germination percentage, seedlings length and weight, and pigments content in leaves) were observed for 20% and 80% extracts. At the same stage, germination of seeds exposed to SMF (exposure times for 3 and 12 min and magnetic inductions of 250 and 500 mT) was studied. The best developed seedlings were determined for the group treated at 3 min with a magnetic induction of 250 mT. In the final step, the simultaneous effects of 20% and 80% algal extracts and treatment with 3 min at 250 mT SMF were tested. Taking into account all the parameters, the simultaneous use of 20% extract and 3 min of 250 mT magnetic induction is recommended.

Keywords

• soybean seeds
• <i>Cladophora glomerata</i>
• algal extract
• static magnetic field
• germination