Química Nova (Jan 2009)

Method validation and stability study of quercetin in topical emulsions

  • Rúbia Casagrande,
  • Marcela M. Baracat,
  • Sandra R. Georgetti,
  • Waldiceu A. Verri Jr,
  • Fabiana T. M. C. Vicentini,
  • Janice A. Rafael,
  • José R. Jabor,
  • Maria J. V. Fonseca

DOI
https://doi.org/10.1590/S0100-40422009000700041
Journal volume & issue
Vol. 32, no. 7
pp. 1939 – 1942

Abstract

Read online

This study validated a high performance liquid chromatography (HPLC) method for the quantitative evaluation of quercetin in topical emulsions. The method was linear within 0.05 - 200 μg/mL range with a correlation coefficient of 0.9997, and without interference in the quercetin peak. The detection and quantitation limits were 18 and 29 ng/mL, respectively. The intra- and inter-assay precisions presented R.S.D. values lower than 2%. An average of 93% and 94% of quercetin was recovered for non-ionic and anionic emulsions, respectively. The raw material and anionic emulsion, but not non-ionic emulsion, were stable in all storage conditions for one year. The method reported is a fast and reliable HPLC technique useful for quercetin determination in topical emulsions.

Keywords