The intracellular mediation during chronic fluoride intoxication in rats

Abstract

The objective of the study was to evaluate the intracellular cyclase cascade under the conditions of chronic fluoride intoxication in white rats of the Wistar population. Materials and methods. The study was conducted from 2009 to 2014 in the Department of Biochemistry and vivarium of Kharkiv National Medical University MOH, Kharkiv, Ukraine. The study was performed on white rats of the Wistar population. In these rats, we administered intragastric, in the morning, on an empty stomach, a solution of sodium fluoride at a rate of 20 mg/kg of body weight. In this way, the development of fluoride intoxication was simulated. The duration of oral administration of the drug was 1.5 months. The intracellular metabolism was assessed in plasma, liver, kidneys, spleen, and brain cortex. The energy components of the adenylate cyclase system, adenosine monophosphate (AMP), adenosine diphosphate (ADP), adenosine triphosphate (ATP), inorganic phosphorus and ions of Mg2+, guanylate cyclase, phosphodiesterase, were measured. The organs and tissues content of cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP) and absorption of Са2+ by the liver membranes microsomes and synaptosomes of the brain were also determined. Results. The increase in the content of cAMP and a decrease in the level of cGMP were observed in the blood plasma of animals, whereas a decrease in the concentration of cAMP and an increase in the amount of cGMP were observed in the liver, kidneys and spleen. In the cerebral cortex, a decrease in adenylate cyclase activity and cAMP content has been established. At the same time, the activity of guanylate cyclase, phosphodiesterase, and cGMP content increased. Conclusions. Analysis of the absorption of Са2+ membranes by macrosomes of the liver and brain synaptosomes revealed a decrease in basal and К+– stimulated absorption of calcium ions by membrane preparations of nerve cells. In the liver, the К+-stimulated and basal absorption of Са2+ ions was significantly less than in the brain.

Keywords

• intracellular metabolism
• liver macrosomes
• brain synaptosomes
• Wistar white rats