Association of the CHRNA3 rs1051730 single nucleotide polymorphism with lung cancer risk in Iranian patients

Abstract

Introdution: Recent studies identified the 15q14/15q25 chromosomal region as a susceptibility locus to lung cancer. This region includes CHRNA3, CHRNA5 and CHRNB4, genes, which coding for nicotinic acetylcholine receptor subunits in lungs and brain. The relationship between several polymorphisms in this locus and lung cancer risk has been widely investigated. The aim of this study was to survey the association of CHRNA3 rs1051730 C>T polymorphism with the risk of lung cancer in Iranian patients. Methods: In this case-control study 96 lung cancer cases in comparison to 100 healthy controls were investigated for the frequency of rs1051730 C>T polymorphism using PCR-RFLP and DNA sequencing methods. The association between the genotypes and allele frequencies with lung cancer was analyzed using chi square and binary logistic regression by SPSS version 22 software. Results: Allele and genotype frequencies of this SNP was not significantly different between case and control groups. The results of this study indicated no significant association between this polymorphism and lung cancer risk in the population studied (OR = 0.566, 95% CI = 0.239-1.342, P= 0.194). Conclusion: Our results showed no significant association between polymorphism rs1051730 C>T and lung cancer risk in the population studied. However, according to the different results obtained from the populations of other studies, further studies are recommended along with an increase in the sample size.

Keywords

• Lung cancer
• rs1051730
• CHRNA3 gene
• PCR-RFLP.