INFLUENCE OF CHANGESIN AMINO ACID COMPOSITION OF BLOOD PROTEIN HYDROLYSATE ON THE PRODUCTIVITY OF ВНК-21/2-17 CELL LINEAGE AND AMOUNT OF FMDV IMMUNOGENIC COMPONENTS

Abstract

The raw material for blood protein hydrolysate preparation is whole animal blood, its clots and other serum production wastes. The dependence of ВНК-21/2-17 cell lineage productivity on the season of hydrolysate raw material preparation was observed. Cell growth stimulated by blood protein hydrolysate from raw material prepared in summer months 1.2–1.3 times exceeded cell growth stimulated by blood protein hydrolysate prepared in autumn-winter period (differences are significant, p < 0,001). The amount of FMDV immunogenic components from “summer” blood protein hydrolysate 1.3–1.6 exceeded the amount obtained during other seasons of the year (differences are significant, p < 0,001). There was found a direct correlation between BHK-21/2-17 cell lineage productivity, concentration of FMDV immunogenic components and seasonal dynamics of the contents of the following amino acids composing blood protein hydrolysates: alanin, asparagine, valine, histidine, glycine, isoleucine, leucine, lysine, methionine, proline, serine, tyrosine, threonine, tryptophane, and phenylalanine. To increase BHK-21/2-17 cell lineage productivity and concentration of FMDV immunogenic components it was suggested to add some additional amino acids into the culture growth medium for suspension cell culture in autumn-summer period together with blood protein hydrolysate

Keywords

• blood protein hydrolysate
• amino acids
• months of the year
• productivity
• fmdv immunogenic components