Mapping of Quantitative Trait Loci Underlying Nodule Traits in Soybean (Glycine max (L.) Merr.) and Identification of Genes Whose Expression Is Affected by the Sinorhizobium fredii HH103 Effector Proteins NopL and NopT

Hejia Ni; Yang Peng; Jinhui Wang; Jing Wang; Yantong Yuan; Tingting Fu; Zikun Zhu; Jialin Zhang; Xipeng Pan; Zhuoling Cui; Chunyan Liu; Qingshan Chen; Dawei Xin

doi:10.3390/agronomy12040946

Agronomy (Apr 2022)

Mapping of Quantitative Trait Loci Underlying Nodule Traits in Soybean (Glycine max (L.) Merr.) and Identification of Genes Whose Expression Is Affected by the Sinorhizobium fredii HH103 Effector Proteins NopL and NopT

Hejia Ni,
Yang Peng,
Jinhui Wang,
Jing Wang,
Yantong Yuan,
Tingting Fu,
Zikun Zhu,
Jialin Zhang,
Xipeng Pan,
Zhuoling Cui,
Chunyan Liu,
Qingshan Chen,
Dawei Xin

Affiliations

Hejia Ni: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China
Yang Peng: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China
Jinhui Wang: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China
Jing Wang: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China
Yantong Yuan: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China
Tingting Fu: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China
Zikun Zhu: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China
Jialin Zhang: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China
Xipeng Pan: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China
Zhuoling Cui: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China
Chunyan Liu: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China
Qingshan Chen: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China
Dawei Xin: Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, China

DOI: https://doi.org/10.3390/agronomy12040946
Journal volume & issue: Vol. 12, no. 4
p. 946

Abstract

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Symbiotic nitrogen fixation provides most of the nitrogen required for soybean growth. Rhizobial nodulation outer proteins (Nops) have been reported to influence host specificity during symbiosis establishment. However, the host proteins that interact with Nops remain unknown. In this study, we generated Sinorhizobium fredii HH103 mutants (HH103ΩNopL, HH103ΩNopT, and HH103ΩNopLΩNopT) and analysed the nodule number (NN) and nodule dry weight (NDW) of 12 soybean germplasms after inoculation with wild-type S. fredii HH103 or the mutant strains. The analysis of chromosome segment substitution lines revealed quantitative trait loci (QTLs) associated with NopL and NopT interactions. A total of 22 QTLs for the 2 nodule traits were detected and mapped to 12 different chromosomes in the soybean genome. Eight and fifteen QTLs were found to be associated with NN and NDW, respectively. Furthermore, 17 candidate genes were selected for further analyses. Considering the results of reverse-transcription quantitative PCR, we propose that the protein products of these 17 candidate genes interact with NopL and NopT.

Published in Agronomy

ISSN: 2073-4395 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture
Website: http://www.mdpi.com/journal/agronomy

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