UVC inactivation of pathogenic samples suitable for cryo-EM analysis

Jamie S. Depelteau; Ludovic Renault; Nynke Althof; C. Keith Cassidy; Luiza M. Mendonça; Grant J. Jensen; Guenter P. Resch; Ariane Briegel

doi:10.1038/s42003-021-02962-w

Communications Biology (Jan 2022)

UVC inactivation of pathogenic samples suitable for cryo-EM analysis

Jamie S. Depelteau,
Ludovic Renault,
Nynke Althof,
C. Keith Cassidy,
Luiza M. Mendonça,
Grant J. Jensen,
Guenter P. Resch,
Ariane Briegel

Affiliations

Jamie S. Depelteau: Department of Microbial Sciences, Institute of Biology, Leiden University
Ludovic Renault: Netherlands Centre for Electron Nanoscopy (NeCEN), Leiden University
Nynke Althof: Department of Microbial Sciences, Institute of Biology, Leiden University
C. Keith Cassidy: Department of Biochemistry, University of Oxford
Luiza M. Mendonça: Biology and Bioengineering Department, California Institute of Technology
Grant J. Jensen: Biology and Bioengineering Department, California Institute of Technology, Pasadena, CA, USA and Department of Chemistry and Biochemistry, Brigham Young University
Guenter P. Resch: Nexperion e.U.–Solutions for Electron Microscopy
Ariane Briegel: Department of Microbial Sciences, Institute of Biology, Leiden University

DOI: https://doi.org/10.1038/s42003-021-02962-w
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 8

Abstract

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Depelteau et al. present a new method to inactivate cryo-EM samples from pathogenic organisms before imaging using ultraviolet-C radiation in cryogenic conditions. This method allows for the inexpensive preparation of cryo-EM samples with no discernable structural impact of the treatment.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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