Наукові горизонти (Jan 2020)
THE PATHOGENIC BEE ENTEROBACTERIA RESISTANCE TO THE EXPERIMENTAL IODINE-CONTAINING DISINFECTANT "JODIS DES NO. 2"
Abstract
The activity of experimental disinfectant against pathogenic bee enterobacteria is shown in the article. Since the use of antibiotics in beekeeping is prohibited in Ukraine, the main task for epizootologists is to prevent the occurrence of infectious bees diseases. The use of new disinfectants and increased veterinary measures on apiaries will contribute to the prevention of bees enterobacteriosis. It has been established that the main causative agents of bee enterobacteriosis are bacteria of the species Klebsiella Pneumoniae, Enterobacter Aerogenes. The aim of our work was to determine the effect of iodine-containing disinfectant on bee cultures of enterobacteriaceae. We found that the disinfectant under study has a significant bacteriostatic effect on Klebsiella Pneumoniae microorganisms at concentrations from 1:100 to native, and a slight bactericidal effect at concentrations from 1:10 to native state. It was also investigated that the present agent has a bacteriostatic effect on Enterobacter Aerogenes microorganisms at concentrations from native state to 1:10, but no bactericidal effect was found. It is known that an important condition for disinfectants is to maintain its activity for a long time. That`s why the next stage of the research was to determine the resistance of the disinfectant depending on the storage time of the drug. We found out that after 14 days of storage of the disinfectant its antimicrobial activity plummets, especially to microorganisms of Enterobacter Aerogenes species, where both bacteriostatic and bactericidal activity were absent. And on bacteria of the Klebsiella Pneumoniae species the drug retained a slight bacteriostatic effect and the diameter of the growth inhibition zone was 10 and 8 mm (in dilutions from native to 1:5). It was shown that the experimental disinfectant effect had both bactericidal and bacteriostatic effects, but it is insufficient for the beehives, frames and beekeeping equipment disinfection in case of enterobacteriosis on apiaries. The activity of the major disinfectant components needs to be improved and investigated. We also recommend to pay attention to the factors that determine the antimicrobial potency of iodine and selenium compounds.
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