Frontiers in Microbiology (May 2022)
Oral Vaccination of Mice With Trichinella spiralis Putative Serine Protease and Murine Interleukin-4 DNA Delivered by Invasive Lactiplantibacillus plantarum Elicits Protective Immunity
- Ying Xue,
- Ying Xue,
- Ying Xue,
- Bo Zhang,
- Bo Zhang,
- Bo Zhang,
- Nan Wang,
- Nan Wang,
- Nan Wang,
- Hai-Bin Huang,
- Hai-Bin Huang,
- Hai-Bin Huang,
- Yu Quan,
- Yu Quan,
- Yu Quan,
- Hui-Nan Lu,
- Hui-Nan Lu,
- Hui-Nan Lu,
- Zhi-Yu Zhu,
- Zhi-Yu Zhu,
- Zhi-Yu Zhu,
- Jun-Yi Li,
- Jun-Yi Li,
- Jun-Yi Li,
- Tian-Xu Pan,
- Tian-Xu Pan,
- Tian-Xu Pan,
- Yue Tang,
- Yue Tang,
- Yue Tang,
- Yan-Long Jiang,
- Yan-Long Jiang,
- Yan-Long Jiang,
- Chun-Wei Shi,
- Chun-Wei Shi,
- Chun-Wei Shi,
- Gui-Lian Yang,
- Gui-Lian Yang,
- Gui-Lian Yang,
- Chun-Feng Wang,
- Chun-Feng Wang,
- Chun-Feng Wang
Affiliations
- Ying Xue
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Ying Xue
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Ying Xue
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Bo Zhang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Bo Zhang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Bo Zhang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Nan Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Nan Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Nan Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Hai-Bin Huang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Hai-Bin Huang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Hai-Bin Huang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yu Quan
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yu Quan
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Yu Quan
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Hui-Nan Lu
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Hui-Nan Lu
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Hui-Nan Lu
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Zhi-Yu Zhu
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Zhi-Yu Zhu
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Zhi-Yu Zhu
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Jun-Yi Li
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Jun-Yi Li
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Jun-Yi Li
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Tian-Xu Pan
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Tian-Xu Pan
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Tian-Xu Pan
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yue Tang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yue Tang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Yue Tang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yan-Long Jiang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yan-Long Jiang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Yan-Long Jiang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Chun-Wei Shi
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Chun-Wei Shi
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Chun-Wei Shi
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Gui-Lian Yang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Gui-Lian Yang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Gui-Lian Yang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Chun-Feng Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Chun-Feng Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Chun-Feng Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- DOI
- https://doi.org/10.3389/fmicb.2022.859243
- Journal volume & issue
-
Vol. 13
Abstract
Trichinellosis is a serious zoonotic parasitic disease caused by Trichinella spiralis (T. spiralis) that causes considerable economic losses for the global pig breeding and food industries. As such, there is an urgent need for a vaccine that can prevent T. spiralis infection. Previous studies have reported that recombinant invasive Lactococcus lactis (LL) expressing Staphylococcus aureus fibronectin binding protein A (LL-FnBPA+) can transfer DNA vaccines directly to dendritic cells (DCs) across an epithelial cell monolayer, leading to significantly higher amounts of heterologous protein expression compared to non-invasive Lactococcus lactis. In this study, the invasive bacterium Lactiplantibacillus plantarum (L. plantarum) expressing FnBPA was used as a carrier to deliver a novel oral DNA vaccine consisting of T. spiralis adult putative serine protease (Ts-ADpsp) and murine interleukin (IL)-4 DNA to mouse intestinal epithelial cells. Experimental mice were orally immunized 3 times at 10-day intervals. At 10 days after the last vaccination, mice were challenged with 350 T. spiralis infective larvae by oral inoculation. Immunization with invasive L. plantarum harboring pValac-Ts-ADpsp/pSIP409-FnBPA induced the production of anti-Ts-ADpsp-specific IgG of serum, type 1 and 2 helper T cell cytokines of mesenteric lymph node (MLN) and spleen, secreted (s) IgA of intestinal lavage, and decreased T. spiralis burden and intestinal damage compared to immunization with non-invasive L. plantarum expressing Ts-ADpsp (pValac-Ts-ADpsp/pSIP409). Thus, invasive L. plantarum expressing FnBPA and IL-4 stimulates both mucosal and cellular immune response to protect against T. spiralis infection, highlighting its therapeutic potential as an effective DNA vaccine for trichinellosis.
Keywords
