Communications Biology (Nov 2022)

Mito-SiPE is a sequence-independent and PCR-free mtDNA enrichment method for accurate ultra-deep mitochondrial sequencing

  • Darren J. Walsh,
  • David J. Bernard,
  • Faith Pangilinan,
  • Madison Esposito,
  • Denise Harold,
  • Anne Parle-McDermott,
  • Lawrence C. Brody

DOI
https://doi.org/10.1038/s42003-022-04182-2
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 11

Abstract

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Mito-SiPE is a mitochondrial DNA (mtDNA) enrichment method that can derive high-quality mtDNA for rare heteroplasmy analysis, without relying on PCR or probes.