The Mitochondrial Import Complex MIM Functions as Main Translocase for α-Helical Outer Membrane Proteins

Abstract

Summary: The mitochondrial outer membrane contains integral proteins with α-helical membrane anchors or a transmembrane β-barrel. The translocase of the outer membrane (TOM) cooperates with the sorting and assembly machinery (SAM) in the import of β-barrel proteins, whereas the mitochondrial import (MIM) complex inserts precursors of multi-spanning α-helical proteins. Single-spanning proteins constitute more than half of the integral outer membrane proteins; however, their biogenesis is poorly understood. We report that the yeast MIM complex promotes the insertion of proteins with N-terminal (signal-anchored) or C-terminal (tail-anchored) membrane anchors. The MIM complex exists in three dynamic populations. MIM interacts with TOM to accept precursor proteins from the receptor Tom70. Free MIM complexes insert single-spanning proteins that are imported in a Tom70-independent manner. Finally, coupling of MIM and SAM promotes early assembly steps of TOM subunits. We conclude that the MIM complex is a major and versatile protein translocase of the mitochondrial outer membrane. : Doan et al. report that the mitochondrial import (MIM) complex constitutes the major import site for single-spanning and multi-spanning outer membrane proteins. MIM exists in three forms and dynamically cooperates with outer membrane protein translocases to import different types of precursor proteins. Keywords: MIM complex, mitochondria, outer membrane, protein assembly, protein sorting, protein translocase, SAM complex, TOM complex