A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae

Yueping Zhang; Juan Wang; Zibai Wang; Yiming Zhang; Shuobo Shi; Jens Nielsen; Zihe Liu

doi:10.1038/s41467-019-09005-3

Nature Communications (Mar 2019)

A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae

Yueping Zhang,
Juan Wang,
Zibai Wang,
Yiming Zhang,
Shuobo Shi,
Jens Nielsen,
Zihe Liu

Affiliations

Yueping Zhang: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology
Juan Wang: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology
Zibai Wang: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology
Yiming Zhang: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology
Shuobo Shi: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology
Jens Nielsen: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology
Zihe Liu: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology

DOI: https://doi.org/10.1038/s41467-019-09005-3
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

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Strain engineering is increasingly the bottleneck step in synthetic biology workflows. Here the authors present GTR-CRISPR for rapid, multiplexed engineering of yeast metabolic pathways.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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