Studying the process of absorption and separation of amino acids (Arg and Glu) by tetraethoxyortho-silane exogel adsorbent

Abstract

In this research, the ability of tetraethoxyorthosilane (TEOS) exogel as a cheap, safe adsorbent with a strong mechanical structure was studied in the separation of acidic (.Glu) and basic (Arg.) amino acids in an absorption chromatography column. First, the effect of pH and then The effect of the length of the chromatography column as well as the hydrophobicity factor of the adsorbent surface on the amount and speed of purification was evaluated. According to the results, the separation rate of the two tested amino acid samples was better at neutral pH (7). In the following, by preparing ormucil derivatives of exogel absorbent (TEOS + Me, TEOS + Et and TEOS + Pro) and testing on the aforementioned amino acids sample, it was found that the effect of the hydrophobicity of the substrate surface is not significant compared to the effect of the pH factor. The length of the chromatography column was effective on the better separation of the above samples.

Keywords

• tetraethoxyorthosilane exogel (teos)
• ormusil
• amino acid
• absorption