Characterization of the complete mitochondrial genome of Caryopemon giganteus Pic (Coleoptera: Chrysomelidae: Bruchinae)

Abstract

We sequenced, assembled, and annotated the complete mitochondrial genome of the seed beetle Caryopemon giganteus, which represents the first report in the tribe Caryopemini from the subfamily Bruchinae of Chrysomelidae. The circular mitochondrial genome of the species contains 15,727 bases, 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and a non-coding region. The GC content of the genome is 25.3%, which is higher than any other reported mitochondrial genomes within Bruchinae. The 16S ribosomal RNA gene and the 12S ribosomal RNA gene are 1284 and 835 bp in length, respectively. 12 PCGs started with the typical ATN codon, except for ND1 initiated with TTG. Five PCGs have the typical stop codon of TAA or TGA, while the remainder PCGs are terminated with incomplete stop codons (TA or T). The phylogenetic analysis based on a combination of 13 genes of the mitochondrial genomes of six species of Bruchinae and 23 species from other 10 subfamilies of Chrysomelidae recovered a generally well resolved and strongly supported tree topology, which shows that C. giganteus has the basalmost position in Bruchinae.

Keywords

• bruchinae
• leguminosae
• mucuna
• phylogeny
• seed beetle