Efficient CRISPR-Cas9 based cytosine base editors for phytopathogenic bacteria

Chenhao Li; Longfei Wang; Leland J. Cseke; Fernanda Vasconcelos; Jose Carlos Huguet-Tapia; Walter Gassmann; Laurens Pauwels; Frank F. White; Hansong Dong; Bing Yang

doi:10.1038/s42003-023-04451-8

Communications Biology (Jan 2023)

Efficient CRISPR-Cas9 based cytosine base editors for phytopathogenic bacteria

Chenhao Li,
Longfei Wang,
Leland J. Cseke,
Fernanda Vasconcelos,
Jose Carlos Huguet-Tapia,
Walter Gassmann,
Laurens Pauwels,
Frank F. White,
Hansong Dong,
Bing Yang

Affiliations

Chenhao Li: Division of Plant Science and Technology, Bond Life Sciences Center, University of Missouri
Longfei Wang: Division of Plant Science and Technology, Bond Life Sciences Center, University of Missouri
Leland J. Cseke: Division of Plant Science and Technology, Bond Life Sciences Center, University of Missouri
Fernanda Vasconcelos: Division of Plant Science and Technology, Bond Life Sciences Center, University of Missouri
Jose Carlos Huguet-Tapia: Department of Plant Pathology, University of Florida
Walter Gassmann: Division of Plant Science and Technology, Bond Life Sciences Center, University of Missouri
Laurens Pauwels: Department of Plant Biotechnology and Bioinformatics, Ghent University
Frank F. White: Department of Plant Pathology, University of Florida
Hansong Dong: Department of Plant Pathology, Nanjing Agricultural University
Bing Yang: Division of Plant Science and Technology, Bond Life Sciences Center, University of Missouri

DOI: https://doi.org/10.1038/s42003-023-04451-8
Journal volume & issue: Vol. 6, no. 1
pp. 1 – 10

Abstract

Read online

A versatile cytosine base editor system is developed to achieve highly efficient C to T conversion in the genome of a series of phytopathogenic bacteria, including Xanthomonas, Pseudomonas, Erwinia, and Agrobacterium.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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