Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

Peter A. Summers; Benjamin W. Lewis; Jorge Gonzalez-Garcia; Rosa M. Porreca; Aaron H. M. Lim; Paolo Cadinu; Nerea Martin-Pintado; David J. Mann; Joshua B. Edel; Jean Baptiste Vannier; Marina K. Kuimova; Ramon Vilar

doi:10.1038/s41467-020-20414-7

Nature Communications (Jan 2021)

Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

Peter A. Summers,
Benjamin W. Lewis,
Jorge Gonzalez-Garcia,
Rosa M. Porreca,
Aaron H. M. Lim,
Paolo Cadinu,
Nerea Martin-Pintado,
David J. Mann,
Joshua B. Edel,
Jean Baptiste Vannier,
Marina K. Kuimova,
Ramon Vilar

Affiliations

Peter A. Summers: Department of Chemistry, Molecular Sciencess Research Hub, White City Campus, Imperial College London
Benjamin W. Lewis: Department of Chemistry, Molecular Sciencess Research Hub, White City Campus, Imperial College London
Jorge Gonzalez-Garcia: Department of Chemistry, Molecular Sciencess Research Hub, White City Campus, Imperial College London
Rosa M. Porreca: Telomere Replication and Stability group, Medical Research Council – London Institute of Medical Sciences
Aaron H. M. Lim: Department of Chemistry, Molecular Sciencess Research Hub, White City Campus, Imperial College London
Paolo Cadinu: Department of Chemistry, Molecular Sciencess Research Hub, White City Campus, Imperial College London
Nerea Martin-Pintado: Oncode Institute, Hubrecht Institute–KNAW and University Medical Center Utrecht
David J. Mann: Department of Life Sciences, Imperial College London South Kensington
Joshua B. Edel: Department of Chemistry, Molecular Sciencess Research Hub, White City Campus, Imperial College London
Jean Baptiste Vannier: Telomere Replication and Stability group, Medical Research Council – London Institute of Medical Sciences
Marina K. Kuimova: Department of Chemistry, Molecular Sciencess Research Hub, White City Campus, Imperial College London
Ramon Vilar: Department of Chemistry, Molecular Sciencess Research Hub, White City Campus, Imperial College London

DOI: https://doi.org/10.1038/s41467-020-20414-7
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 11

Abstract

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Direct observation of G-quadruplexes (G4s) in live cells is challenging. Here the authors report a method to identify G4s within the nuclei of live and fixed cells using a fluorescent probe combined with fluorescence lifetime imaging microscopy.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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