Bioactive Materials (Oct 2022)
Antibiofilm and immunomodulatory resorbable nanofibrous filing for dental pulp regenerative procedures
- Mauricio Gonçalves da Costa Sousa,
- Gabriela Conceição de Almeida,
- Danilo César Martins Mota,
- Rosiane Andrade da Costa,
- Simoni Campos Dias,
- Samuel Nunes Limberger,
- Frank Ko,
- Li Ting Lin,
- Evan F. Haney,
- Hashem Etayash,
- Beverlie Baquir,
- Michael J. Trimble,
- Ya Shen,
- Zheng Su,
- Markus Haapasalo,
- Daniel Pletzer,
- Letícia Chaves de Souza,
- Gláucia Schuindt Teixeira,
- Renato M. Silva,
- Robert E.W. Hancock,
- Octavio Luiz Franco,
- Taia Maria Berto Rezende
Affiliations
- Mauricio Gonçalves da Costa Sousa
- Post-Graduation Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, Brazil
- Gabriela Conceição de Almeida
- Dentistry Course, Catholic University of Brasília, Brasília, Brazil
- Danilo César Martins Mota
- Post-Graduation Program in Health Sciences, University of Brasília, Brazil
- Rosiane Andrade da Costa
- Post-Graduation Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, Brazil
- Simoni Campos Dias
- Post-Graduation Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, Brazil; Animal Biology Department, Campus Darcy Ribeiro, Universidade de Brasília, Brazil
- Samuel Nunes Limberger
- LIMA, Chemistry Institute, University of Brasília-UNB, Brasília, Brazil
- Frank Ko
- Department of Materials Engineering, Faculty of Applied Science, University of British Columbia, Vancouver, Canada
- Li Ting Lin
- Department of Materials Engineering, Faculty of Applied Science, University of British Columbia, Vancouver, Canada
- Evan F. Haney
- Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada
- Hashem Etayash
- Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada
- Beverlie Baquir
- Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada
- Michael J. Trimble
- Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada
- Ya Shen
- Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, Canada
- Zheng Su
- Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, Canada
- Markus Haapasalo
- Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, Canada
- Daniel Pletzer
- Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand
- Letícia Chaves de Souza
- Department of Endodontics, School of Dentistry, University of Texas Health Science Center at Houston, Houston, USA
- Gláucia Schuindt Teixeira
- Department of Endodontics, School of Dentistry, University of Texas Health Science Center at Houston, Houston, USA; Department of Prosthesis, Faculty of Dentistry, Rio de Janeiro State University, Rio de Janeiro, Brazil
- Renato M. Silva
- Department of Endodontics, School of Dentistry, University of Texas Health Science Center at Houston, Houston, USA
- Robert E.W. Hancock
- Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada
- Octavio Luiz Franco
- Post-Graduation Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, Brazil; Post-Graduation Program in Biotechnology, Catholic University Dom Bosco, Campo Grande, Mato Grosso do Sul, Brazil
- Taia Maria Berto Rezende
- Post-Graduation Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, Brazil; Dentistry Course, Catholic University of Brasília, Brasília, Brazil; Post-Graduation Program in Health Sciences, University of Brasília, Brazil; Corresponding author. Universidade Católica de Brasília, Pós-graduação em Ciências Genômicas e Biotecnologia, SGAN 916N – Av. W5 – Campus II – Modulo C, room C-221, Brasília-DF, Brazil.
- Journal volume & issue
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Vol. 16
pp. 173 – 186
Abstract
Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry. Here, different scaffolds were tested for use during the pulp revascularization process, including poly(vinyl alcohol)-PVA hydrogels or resins, collagen hydrogels and poly(vinyl alcohol) PVA/Chitosan (PVA/CS) nanofibers. Based on time to degradation (21 days), nanofibers were chosen to be incorporated with ciprofloxacin and IDR-1002 (each at 50 mg/g). Nanofibers containing ciprofloxacin and IDR-1002 had anti-biofilm activity against Enterococcus faecalis, Staphylococcus aureus and a multispecies oral biofilm, besides anti-inflammatory activities. The in vivo subcutaneous tissue response to tooth fragments filled with nanofibers demonstrated a pulp-like tissue formation, when compared to empty teeth fragments. Thus, we designed a strong antimicrobial, immunomodulatory and regenerative candidate for pulp revascularization and regeneration procedures.
