Parasite Epidemiology and Control (Aug 2021)
Relative abundance and molecular identification of Culex pipiens complex (Diptera: Culicidae), in Kura Local Government Area, North-western Nigeria
Abstract
Culex species are the most widespread mosquito species across the world and are known to be highly opportunistic, feeding on humans and livestock. They are known to acquire the potential to transmit zoonotic diseases, including Rift Valley Fever (RVF). However, despite their public health significance, they remain understudied in North-western Nigeria, compared to Anophelines. This study was therefore aimed at determining the relative abundance and Multiplex polymerase chain reaction (Multiplex PCR) identification of members of the Culex pipiens complex, in Kura Local Government Area (LGA), North-western, Nigeria. Adult mosquitoes were collected using Center for Disease Control (CDC) miniature light traps from August to October 2019. Mosquitoes were identified using morphological identification keys. Members of the Culex pipiens complex were further identified using Multiplex PCR to assess the presence of sibling species. A total of 413 mosquitoes, belonging to 3 genera, Culex, Anopheles and Aedes were collected. Of this figure, 120 Culex spp. females were collected. Homes with livestock had the highest occurrence of mosquitoes, 123 (61.19%) compared to those without livestock, 78 (38.81%). There was no statistical difference among the two (2) categories of homes (P ≥ 0.005). Culicoides spp. were the most common with 130 collected (65.38%). Again, homes with livestock had the highest occurrence, 85 whilst homes without livestock had 45 of the other flies caught. Multiplex- PCR revealed no expected bands for Cx. quinquefasciatus and Cx. pipiens from the DNA obtained from field collected mosquitoes as confirmed by using genomic DNA of an insectary Culex quinquefasciatus as control. Cx. spp. is presently regarded as a biting nuisance having no significant epidemiological importance. Efforts at its control should be intensified before it is too late. This study provides useful information on the occurrence and multiplex PCR of Culex spp in Kura Local Government Area, North-western Nigeria. These results have implications for the control of Culex spp. mosquito populations and the spread of human, livestock and avian diseases.
