eJHaem (Nov 2021)

Artemisinins induce endoplasmic reticulum stress in acute leukaemia cells in vitro and in vivo

  • Rubia Isler Mancuso,
  • Juliana Hofstätter Azambuja,
  • Fernanda Soares Niemann,
  • Ada Congrains,
  • Mary Ann Foglio,
  • Eduardo Magalhães Rego,
  • Sara Teresinha Olalla Saad

DOI
https://doi.org/10.1002/jha2.314
Journal volume & issue
Vol. 2, no. 4
pp. 818 – 822

Abstract

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Abstract Loss of endoplasmic reticulum (ER) homeostasis leads to ER stress, thus prolonged activation can lead to apoptosis. Herein, artesunate (ART) induced ER stress in leukaemia cells, resulting in eIF2α phosphorylation, activation of transcription factor 4, subsequent CHOP upregulation and XBP1 splicing. Furthermore, in vitro cyclin/CDKs reduction induced G1‐phase arrest. An in vivo xenograft model showed a consistent pattern of ART in reducing tumour burden, supporting roles in the UPR pathway, which we speculate could lead to apoptosis by NOXA activation. Moreover, ART were capable of increasing the survival of mice. Taken together, our data indicate that ART may represent an interesting weapon to fight leukaemia.

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