Frontiers in Microbiology (Mar 2023)
CDetection.v2: One-pot assay for the detection of SARS-CoV-2
- Xinge Wang,
- Xinge Wang,
- Xinge Wang,
- Xinge Wang,
- Yangcan Chen,
- Yangcan Chen,
- Yangcan Chen,
- Yangcan Chen,
- Xuejia Cheng,
- Si-Qi Wang,
- Si-Qi Wang,
- Si-Qi Wang,
- Yanping Hu,
- Yanping Hu,
- Yanping Hu,
- Yanping Hu,
- Yingmei Feng,
- Ronghua Jin,
- Kangping Zhou,
- Ti Liu,
- Jianxing Wang,
- Kai Pan,
- Bing Liu,
- Jie Xiang,
- Yanping Wang,
- Qi Zhou,
- Qi Zhou,
- Qi Zhou,
- Qi Zhou,
- Ying Zhang,
- Ying Zhang,
- Ying Zhang,
- Weiye Pan,
- Wei Li,
- Wei Li,
- Wei Li,
- Wei Li
Affiliations
- Xinge Wang
- State Key Laboratory of Stem Cell and Reproductive Biology, Chinese Academy of Sciences, Institute of Zoology, Beijing, China
- Xinge Wang
- Bejing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Xinge Wang
- Chinese Academy of Sciences, Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Xinge Wang
- Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China
- Yangcan Chen
- State Key Laboratory of Stem Cell and Reproductive Biology, Chinese Academy of Sciences, Institute of Zoology, Beijing, China
- Yangcan Chen
- Bejing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Yangcan Chen
- Chinese Academy of Sciences, Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Yangcan Chen
- Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China
- Xuejia Cheng
- Beijing SynsorBio Technology Co., Ltd., Beijing, China
- Si-Qi Wang
- State Key Laboratory of Stem Cell and Reproductive Biology, Chinese Academy of Sciences, Institute of Zoology, Beijing, China
- Si-Qi Wang
- Bejing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Si-Qi Wang
- Chinese Academy of Sciences, Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Yanping Hu
- State Key Laboratory of Stem Cell and Reproductive Biology, Chinese Academy of Sciences, Institute of Zoology, Beijing, China
- Yanping Hu
- Bejing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Yanping Hu
- Chinese Academy of Sciences, Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Yanping Hu
- Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China
- Yingmei Feng
- Department of Science and Technology, Beijing Youan Hospital, Capital Medical University, Beijing, China
- Ronghua Jin
- Beijing Ditan Hospital, Capital Medical University, Beijing, China
- Kangping Zhou
- Hubei Provincial Center for Disease Control and Prevention, Wuhan, China
- Ti Liu
- Shandong Center for Disease Control and Prevention, Jinan, China
- Jianxing Wang
- Shandong Center for Disease Control and Prevention, Jinan, China
- Kai Pan
- Hubei Provincial Center for Disease Control and Prevention, Wuhan, China
- Bing Liu
- 0Tonghua Central Hospital, Tonghua, Jilin, China
- Jie Xiang
- 1Tongji Medical College of Huazhang, Wuhan Jinyintan Hospital, University of Science and Technology, Wuhan, China
- Yanping Wang
- 2Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China
- Qi Zhou
- State Key Laboratory of Stem Cell and Reproductive Biology, Chinese Academy of Sciences, Institute of Zoology, Beijing, China
- Qi Zhou
- Bejing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Qi Zhou
- Chinese Academy of Sciences, Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Qi Zhou
- Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China
- Ying Zhang
- State Key Laboratory of Stem Cell and Reproductive Biology, Chinese Academy of Sciences, Institute of Zoology, Beijing, China
- Ying Zhang
- Bejing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Ying Zhang
- Chinese Academy of Sciences, Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Weiye Pan
- Beijing SynsorBio Technology Co., Ltd., Beijing, China
- Wei Li
- State Key Laboratory of Stem Cell and Reproductive Biology, Chinese Academy of Sciences, Institute of Zoology, Beijing, China
- Wei Li
- Bejing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Wei Li
- Chinese Academy of Sciences, Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Wei Li
- Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China
- DOI
- https://doi.org/10.3389/fmicb.2023.1158163
- Journal volume & issue
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Vol. 14
Abstract
IntroductionThe ongoing 2019 coronavirus disease pandemic (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its variants, is a global public health threat. Early diagnosis and identification of SARS-CoV-2 and its variants plays a critical role in COVID-19 prevention and control. Currently, the most widely used technique to detect SARS-CoV-2 is quantitative reverse transcription real-time quantitative PCR (RT-qPCR), which takes nearly 1 hour and should be performed by experienced personnel to ensure the accuracy of results. Therefore, the development of a nucleic acid detection kit with higher sensitivity, faster detection and greater accuracy is important.MethodsHere, we optimized the system components and reaction conditions of our previous detection approach by using RT-RAA and Cas12b.ResultsWe developed a Cas12b-assisted one-pot detection platform (CDetection.v2) that allows rapid detection of SARS-CoV-2 in 30 minutes. This platform was able to detect up to 5,000 copies/ml of SARS-CoV-2 without cross-reactivity with other viruses. Moreover, the sensitivity of this CRISPR system was comparable to that of RT-qPCR when tested on 120 clinical samples.DiscussionThe CDetection.v2 provides a novel one-pot detection approach based on the integration of RT-RAA and CRISPR/Cas12b for detecting SARS-CoV-2 and screening of large-scale clinical samples, offering a more efficient strategy for detecting various types of viruses.
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