Immunohistochemical localization of catalase in geese (anser anser) kidney

Abstract

The aim of this study is to observe the histologic structure of kidney tissue of geese and to determine the immunohistochemical localization of catalase in it. Six female geese were used in our study. After fixation in Bouin"s solution and routine histological process, the kidney tissues taken from geese were embedded in paraffin blocks. To detect catalase immunoreactivity in the tissues, the avidinbiotin- peroxidase complex (ABC) technique was used. Crossmann"s modified triple stain for histological examination was applied to the samples. It was seen that the geese kidney included numerous lobules and each lobule consists of cortical and medullary tissues. The strong immunoreactivity of catalase was observed in the proximal convoluted tubules in the cortex. Immunohistochemical reaction was also observed in intermediate tubules and the thick segment of Henle"s loops. Also it was determined that the immunoreactivity in the basal epithelial cells of the secondary branches of ureters and the intercalated cells in the distal convoluted tubules and collecting duct. There was no catalase immunoreactivity in the renal corpuscles. In conclusion, localization of catalase in the proximal convoluted tubules, in the intermediate tubules, in the thick segments of Henle"s loops and in the intercalated cells is considered that these parts of the geese kidney are involved in the antioxidant defence. This study is thought to contribute to the planned future studies about antioxidant system in the kidney of poultry.

Keywords

• catalase
• goose
• kidney
• immunohistochemistry