Nature Communications (Sep 2017)
pGlyco 2.0 enables precision N-glycoproteomics with comprehensive quality control and one-step mass spectrometry for intact glycopeptide identification
- Ming-Qi Liu,
- Wen-Feng Zeng,
- Pan Fang,
- Wei-Qian Cao,
- Chao Liu,
- Guo-Quan Yan,
- Yang Zhang,
- Chao Peng,
- Jian-Qiang Wu,
- Xiao-Jin Zhang,
- Hui-Jun Tu,
- Hao Chi,
- Rui-Xiang Sun,
- Yong Cao,
- Meng-Qiu Dong,
- Bi-Yun Jiang,
- Jiang-Ming Huang,
- Hua-Li Shen,
- Catherine C. L. Wong,
- Si-Min He,
- Peng-Yuan Yang
Affiliations
- Ming-Qi Liu
- Institutes of Biomedical Sciences and Department of Chemistry, Fudan University
- Wen-Feng Zeng
- Key Lab of Intelligent Information Processing of Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS
- Pan Fang
- Institutes of Biomedical Sciences and Department of Chemistry, Fudan University
- Wei-Qian Cao
- Institutes of Biomedical Sciences and Department of Chemistry, Fudan University
- Chao Liu
- Key Lab of Intelligent Information Processing of Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS
- Guo-Quan Yan
- Institutes of Biomedical Sciences and Department of Chemistry, Fudan University
- Yang Zhang
- Institutes of Biomedical Sciences and Department of Chemistry, Fudan University
- Chao Peng
- National Center for Protein Science (Shanghai), Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, CAS
- Jian-Qiang Wu
- Key Lab of Intelligent Information Processing of Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS
- Xiao-Jin Zhang
- Key Lab of Intelligent Information Processing of Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS
- Hui-Jun Tu
- Key Lab of Intelligent Information Processing of Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS
- Hao Chi
- Key Lab of Intelligent Information Processing of Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS
- Rui-Xiang Sun
- Key Lab of Intelligent Information Processing of Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS
- Yong Cao
- National Institute of Biological Sciences (Beijing)
- Meng-Qiu Dong
- National Institute of Biological Sciences (Beijing)
- Bi-Yun Jiang
- Institutes of Biomedical Sciences and Department of Chemistry, Fudan University
- Jiang-Ming Huang
- Institutes of Biomedical Sciences and Department of Chemistry, Fudan University
- Hua-Li Shen
- Institutes of Biomedical Sciences and Department of Chemistry, Fudan University
- Catherine C. L. Wong
- National Center for Protein Science (Shanghai), Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, CAS
- Si-Min He
- Key Lab of Intelligent Information Processing of Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS
- Peng-Yuan Yang
- Institutes of Biomedical Sciences and Department of Chemistry, Fudan University
- DOI
- https://doi.org/10.1038/s41467-017-00535-2
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 14
Abstract
Protein glycosylation is a heterogeneous post-translational modification that generates greater proteomic diversity that is difficult to analyze. Here the authors describe pGlyco 2.0, a workflow for the precise one step identification of intact N-glycopeptides at the proteome scale.
