Enrichment and identification of Δ9-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants

Kerstin Lange; Ansgar Poetsch; Andreas Schmid; Mattijs K. Julsing

doi:10.1016/j.dib.2015.07.033

Data in Brief (Sep 2015)

Enrichment and identification of Δ9-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants

Kerstin Lange,
Ansgar Poetsch,
Andreas Schmid,
Mattijs K. Julsing

Affiliations

Kerstin Lange: Laboratory of Chemical Biotechnology, Department of Biochemical & Chemical Engineering, TU Dortmund University, 44227 Dortmund, Germany
Ansgar Poetsch: Plant Biochemistry, Ruhr University Bochum, 44801 Bochum, Germany
Andreas Schmid: Department of Solar Materials, Helmholtz Centre for Environmental Research (UFZ), 04318 Leipzig, Germany
Mattijs K. Julsing: Laboratory of Chemical Biotechnology, Department of Biochemical & Chemical Engineering, TU Dortmund University, 44227 Dortmund, Germany

DOI: https://doi.org/10.1016/j.dib.2015.07.033
Journal volume & issue: Vol. 4, no. C
pp. 641 – 649

Abstract

Read online

This data article refers to the report Δ9-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P. pastoris KM71 KE1. Enrichment of THCAS as a technically pure enzyme was realized using dialysis and cationic exchange chromatography. nLC-ESI-MS/MS analysis identified THCAS in different fractions obtained by cationic exchange chromatography.

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

About the journal