Earlier Denaturation of DNA By Using Novel Ternary Hybrid Nanoparticles

Abstract

Two novel ternary hybrid nanoparticles (THNp) consisting of graphene oxide (GO) and reduced graphene oxides (rGO) were added to samples of DNA. The effect of the addition of nanoparticles on the thermal denaturation of DNA samples was studied by measuring the absorbance using a temperature-controlled Perkin Elmer UV spectrophotometer. Adding GO-TiO2-Ag and rGO-TiO2-Ag nanoparticles lowered the denaturation temperature of template DNA significantly. The nanoparticles affect the denaturation rate. The optimal GO-TiO2-Ag and rGO-TiO2-Ag concentrations were found to be 5 × 10-2, which resulted in 86- and 180-folds augmentation of DNA denaturation (6.5 µg/mL), respectively, while it resulted in 2- and 7-folds augmentation of DNA denaturation (11.5 µg/mL), respectively, at temperature as low as 80 °C. The results indicated that rGO-TiO2-Ag nanoparticles exhibited significantly higher DNA denaturation enhancement than rGO-TiO2-Ag nanoparticles, owing to their enhanced thermal conductivity effect. Therefore, these nanoparticles could help to get improved PCR yield, hence enable amplification to be performed for longer cycles by lowering the denaturation temperatures. ABSTRAK: Dua ternar baru nanopartikel hibrid (THNp) mengandungi oksida grapen (GO) dan oksida grapen yang dikurangkan (rGO) dan dimasukkan ke dalam sampel DNA. Kesan penambahan nanopartikel pada denaturasi termal pada sampel DNA telah dikaji dengan mengukur penyerapan menggunakan kawalan-suhu Perkin Elmer UV spektrofotometer. Penambahan GO-TiO2-Ag dan rGO-TiO2-Ag nanopartikel telah mengurangkan suhu denaturasi pada templat DNA dengan nyata. Nanopartikel memberi kesan pada kadar denaturasi. Kepekatan optimal GO-TiO2-Ag dan rGO-TiO2-Ag didapati sebanyak 5 × 10-2, menyebabkan penambahan sebanyak 86- dan 180-lipat pada DNA denaturasi (6.5 µg/mL), masing-masing, sementara ia menyebabkan sebanyak 2- dan 7-lipat penambahan pada DNA denaturasi (11.5 µg/mL), masing-masing, pada suhu serendah 80 °C. Dapatan menunjukkan nanopartikel rGO-TiO2-Ag mempunyai kenaikan penambahan DNA denaturasi nyata berbanding nanopartikel rGO-TiO2-Ag, disebabkan kesan kekonduksian penambahan suhu. Oleh itu, nanopartikel ini dapat membantu bagi penambah baikan pengeluaran PCR, membolehkan penguatan dapat dilakukan dalam kitaran lebih lama dengan merendahkan suhu denaturasi.

Keywords

• DNA denaturation
• polymerase chain reaction (PCR)
• nano-PCR
• hybrid nanoparticles