TOXIC EFFECT OF MANGANESE OXIDE NANOPARTICLES IN ACUTE EXPERIMENT

Abstract

It has been established that manganese compounds have a negative effect on a living organism, exhibiting neurotoxic and hepatotoxic effects and inducing oxidative stress. Since the employees of the manganese ore extraction factory as well as the weld engineers, are constantly in contact with manganese nanoparticles, the study of toxic effects of these nanoparticles on living organisms is relevant. The aim of the study – to investigate the toxicity of manganese in the form of nanoparticles compared with equivalent doses of manganese in the form of salt in their oral administration into the body. Materials and Methods. For the experiment, male rats of the Wistar line have been used (3 experimental groups of 8 rats in each). Animals of the first group were intact. A solution of manganese salts and a suspension of nanoparticles has been administered daily to animals of groups ІІ and ІІІ by means of a probe, taking into account a single dose of manganese of 10.9 mg/kg of body weight. For determination of biochemical parameters, sets of reagents of firms "Filisit-Diagnostika" and "SpinLab" have been used. Results and Discussion. The poisoning was simulated in conditions of acute per os administration of manganese oxide nanoparticules suspension and manganese salt solution for 8 days (total dose of manganese 87.2 mg/kg body weight of white rats). It has been shown that the introduction of manganese-containing nanoparticles and manganese salt induced significant changes of biochemical parameters. Conclusions. Introduction of compounds of manganese induces significant changes in the biochemical parameters of blood serum and homogenates, inducing hepatotoxicity and neurotoxicity, as well as oxidative stress. In the administration of equivalent doses of manganese, its toxicity in the form of a nanocompound is higher than in the form of a manganese salt.

Keywords

• наночастинки
• токсичність
• марганець.