Detecting microRNA-mediated gene regulatory effects in murine neuronal subpopulations

Abstract

Summary: microRNAs (miRNAs) have unique gene regulatory effects in different neuronal subpopulations. Here, we describe a protocol to identify neuronal subtype-specific effects of a miRNA in murine motor neuron subpopulations. We detail the preparation of primary mouse spinal tissue for single cell RNA sequencing and bioinformatics analyses of pseudobulk expression data. This protocol applies differential gene expression testing approaches to identify miRNA target networks in heterogeneous neuronal subpopulations that cannot otherwise be captured by bulk RNA sequencing approaches.For complete details on the use and execution of this protocol, please refer to Amin et al. (2021).

Keywords

• Bioinformatics
• Cell isolation
• Single Cell
• Flow Cytometry/Mass Cytometry
• Developmental biology
• Model Organisms