Quantitative analysis of piperidin-1-ium {[5-(2-furyl)-4-phenyl-4h-1,2,4-triazol-3-yl]thio}acetate, substance of the veterinary drug “tryfuzol”, in poultry meat by lc–dad–ms

Abstract

Development of sensitive, acurate, reproducible HPLC-DMD-MS method for determination of the residualamounts of active drug substance "Tryfuzol" in poultryhomogenatesamples and application of this method for testing chiken groups is an important task for the confirmation of thi drug safety. The substance extraction conditions were optimized for methods development. It was established optimum extraction condition. Chromatographic conditions and massspectrometric studies were optimized. As mobilephase, a flowrate of 0.4 ml min-1 isocraticelution 0.1% (v / v) formicacid in water (A) acetonitrile containing 0.1% (v / v) formicacid (B). The composition of the mobile phase of 70% / 30% (v / v). Total running time is 5 minutes. Wave length of the detectoris 276 nm. Control of substances availability was carried by retention time and under UV and massspectra. The technique complies with relevant regulations. The absence of saidsubstance in the study chiken group was proven by using developed techniques.

Keywords

• Piperidin-1-ium 2-{[5-(2-furyl)-4-phenyl-4h-1 2 4-triazol-3-yl]thio}acetate
• poultry meat homogenate
• column liquid chromatography
• single quadrupolemass spectrometry