Russian Journal of Agricultural and Socio-Economic Sciences (Apr 2019)
THE QUALITY OF LANDRACE PIG SPERMATOZOA DURING THE COLD STORAGE PROCESS USING BTS AND CEP-3 DILUENTS ADDED 10% EGG YOLK AT TEMPERATURES OF 2-5ﹾC
Abstract
In East Nusa Tenggara many Landrace pigs are used to fullfil the protein needs of the community and Artificial Insemination has been used to improve the genetic quality of pigs. The objective of this study was to find out the period of cold storage on the quality of the Landrace pig spermatozoa using BTS and CEP-3 diluents and added 10% egg yolk. This research was conducted at the Laboratory of the Faculty of Agriculture of Timor University using Landrace pig spermatozoa. The research method is a laboratory experiment using 2 treatments with 10 replications, namely T1 (BTS) and T2 (CEP-3 + 10% egg yolk) which stored at refrigerator temperature of 2-50C. The variables observed were individual motility and membrane integrity of spermatozoa. Data were analyzed using Randomized Block Design (RBD) Motility percentage using Pearson's ChiSquare with an expected value of 40 million motile sperm/ml. The results showed that the percentage of individual motility at storage period of the 3rd hour type of diluent had no significant effect (P>0.05) whereas at the storage time/period of the 6th, 9th, 24th, 48th and 72nd hours percentage of individual motility T1 with the value of 66.5%, 61.5%, 52.5%, 42.5%, 32% significantly different (P 0.05) on the membrane integrity of Landrace pig spermatozoa. Menwhile at 6th, 9th, 24th, 48th, and 72nd hours showed that the treatment of T1 with a value of 77.68%, 73.59%, 64.61%, 52.71%, and 42.18% respectively gave a significant effect (P <0.05) on T2 with a value of 75.86%, 67.73%, 57.95%, 47.09 % and 33.71% respectively. The conclusion of this study namely diluent that has a higher advantage in maintaining the quality of spermatozoa of Landrace pigs at a temperature of 2-50C is BTS diluent which has the highest percentage of motility compared to CEP-3 diluent + 10% egg yolk. The results showed that BTS diluent can be stored for 48 hours while CEP-3 diluent +10% egg yolk can be stored for 24 hours with a minimum percentage of spermatozoa motility of 40%.
Keywords
