Journal of Lipid Research (Nov 1974)
Analysis of α-lipoprotein cholesterol in 50 μl of plasma
Abstract
A micromethod for quantitation of α-lipoprotein cholesterol was devised for gas–liquid chromatography to minimize plasma sample size and facilitate lipid studies using capillary blood from children or small animals. α-Lipoprotein cholesterol was measured by gas–liquid chromatography in 20 μl of the centrifuged supernate obtained after addition of 5 μl of mixed heparin–manganese chloride solution 1:1 (v/v) to 50 μl of plasma. Comparison of venous α-lipoprotein cholesterol measured by gas–liquid chromatography with venous α-lipoprotein cholesterol measured by conventional heparin–manganese precipitation and ferric chloride (colorimetric) cholesterol determination gave a correlation coefficient of 0.98 for 80 plasma samples. Capillary α-lipoprotein cholesterol and venous α-lipoprotein cholesterol were closely correlated in 31 patients (r = 0.97).
