Development and in-vitro characterization of HAP blended PVA/PEG bio-membrane

Abstract

Hydroxyapatite HAP is a bioceramic that can be applied for bone tissue regeneration. Polyvinyl Alcohol (PVA)/Polyethylene Glycol (PEG) polymers are water-soluble and have faster biodegradation properties. The PVA-PEG-HAp thin membrane was cast by using the transparent solution petri-dish method. The PVA-PEG-HAp thin membrane was investigated by Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Thermogravimetric Analysis (TGA), Energy Dispersive X-Ray Analysis (EDX), and Field Emission Scanning Electron Microscope (FESEM. Both PVA and HAp of membrane matrix had typical peaks at 2Ɵ value 19.78. PEG also has a distinctive peak at 26.687. The five (5, 25, 50, 75, and 10 μl) different concentrations of extracts of PVA-PEG-HAp thin membrane were pertinent to interacting for 24 h with the L929 mouse cell line, and the results were exhibited a maximum of 99% and 93% cell viability, 1% and 7% cytotoxicity, respectively. Hence the newly developed polymer biomembrane is a biocompatible alternative for biofilms in medical applications.

Keywords

• Hydroxyapatite
• PVA
• PEG
• Cytotoxicity
• HAP
• Bio-membrane