Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor

Jian Wang; Yuxi Teng; Ruihua Zhang; Yifei Wu; Lei Lou; Yusong Zou; Michelle Li; Zhong-Ru Xie; Yajun Yan

doi:10.1038/s41467-021-27290-9

Nature Communications (Nov 2021)

Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor

Jian Wang,
Yuxi Teng,
Ruihua Zhang,
Yifei Wu,
Lei Lou,
Yusong Zou,
Michelle Li,
Zhong-Ru Xie,
Yajun Yan

Affiliations

Jian Wang: School of Chemical, Materials and Biomedical Engineering, College of Engineering, University of Georgia
Yuxi Teng: School of Chemical, Materials and Biomedical Engineering, College of Engineering, University of Georgia
Ruihua Zhang: School of Chemical, Materials and Biomedical Engineering, College of Engineering, University of Georgia
Yifei Wu: School of Electrical and Computer Engineering, College of Engineering, University of Georgia
Lei Lou: School of Electrical and Computer Engineering, College of Engineering, University of Georgia
Yusong Zou: School of Chemical, Materials and Biomedical Engineering, College of Engineering, University of Georgia
Michelle Li: North Oconee High School
Zhong-Ru Xie: School of Electrical and Computer Engineering, College of Engineering, University of Georgia
Yajun Yan: School of Chemical, Materials and Biomedical Engineering, College of Engineering, University of Georgia

DOI: https://doi.org/10.1038/s41467-021-27290-9
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 10

Abstract

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SpCas9 has a NGG PAM requirement that limits its DNA target range. Here the authors present a PAM-expanded SpdCas9 variant SpdNG-LWQT to target NRN PAMs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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