Nuclear condensates of p300 formed though the structured catalytic core can act as a storage pool of p300 with reduced HAT activity

Yi Zhang; Kyle Brown; Yucong Yu; Ziad Ibrahim; Mohamad Zandian; Hongwen Xuan; Steven Ingersoll; Thomas Lee; Christopher C. Ebmeier; Jiuyang Liu; Daniel Panne; Xiaobing Shi; Xiaojun Ren; Tatiana G. Kutateladze

doi:10.1038/s41467-021-24950-8

Nature Communications (Jul 2021)

Nuclear condensates of p300 formed though the structured catalytic core can act as a storage pool of p300 with reduced HAT activity

Yi Zhang,
Kyle Brown,
Yucong Yu,
Ziad Ibrahim,
Mohamad Zandian,
Hongwen Xuan,
Steven Ingersoll,
Thomas Lee,
Christopher C. Ebmeier,
Jiuyang Liu,
Daniel Panne,
Xiaobing Shi,
Xiaojun Ren,
Tatiana G. Kutateladze

Affiliations

Yi Zhang: Department of Pharmacology, University of Colorado School of Medicine
Kyle Brown: Department of Chemistry, University of Colorado
Yucong Yu: Center for Epigenetics, Van Andel Research Institute
Ziad Ibrahim: Department of Molecular and Cell Biology, Leicester Institute of Structural and Chemical Biology, University of Leicester
Mohamad Zandian: Department of Pharmacology, University of Colorado School of Medicine
Hongwen Xuan: Center for Epigenetics, Van Andel Research Institute
Steven Ingersoll: Department of Chemistry, University of Colorado
Thomas Lee: Department of Biochemistry, University of Colorado
Christopher C. Ebmeier: Department of Biochemistry, University of Colorado
Jiuyang Liu: Department of Pharmacology, University of Colorado School of Medicine
Daniel Panne: Department of Molecular and Cell Biology, Leicester Institute of Structural and Chemical Biology, University of Leicester
Xiaobing Shi: Center for Epigenetics, Van Andel Research Institute
Xiaojun Ren: Department of Chemistry, University of Colorado
Tatiana G. Kutateladze: Department of Pharmacology, University of Colorado School of Medicine

DOI: https://doi.org/10.1038/s41467-021-24950-8
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 15

Abstract

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The histone acetyltransferase p300 mostly localizes to active chromatin; however, some repressed genes marked with H3K27me3 are also bound by p300. Here the authors show p300 is capable of phase separation, which relies on its catalytic core, and that p300 catalytic activity is decreased in phase-separated droplets that co-localize with H3K27me3-marked chromatin.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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