RNAi-based pest control: Production, application and the fate of dsRNA

Li He; Li He; Li He; Yanna Huang; Yanna Huang; Yanna Huang; Xueming Tang; Xueming Tang; Xueming Tang

doi:10.3389/fbioe.2022.1080576

Frontiers in Bioengineering and Biotechnology (Nov 2022)

RNAi-based pest control: Production, application and the fate of dsRNA

Li He,
Li He,
Li He,
Yanna Huang,
Yanna Huang,
Yanna Huang,
Xueming Tang,
Xueming Tang,
Xueming Tang

Affiliations

Li He: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China
Li He: Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Shanghai, China
Li He: Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Ministry of Science and Technology, Ministry of Education, Shanghai, China
Yanna Huang: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China
Yanna Huang: Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Shanghai, China
Yanna Huang: Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Ministry of Science and Technology, Ministry of Education, Shanghai, China
Xueming Tang: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China
Xueming Tang: Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Shanghai, China
Xueming Tang: Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Ministry of Science and Technology, Ministry of Education, Shanghai, China

DOI: https://doi.org/10.3389/fbioe.2022.1080576
Journal volume & issue: Vol. 10

Abstract

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The limitations of conventional pesticides have raised the demand for innovative and sustainable solutions for plant protection. RNA Interference (RNAi) triggered by dsRNA has evolved as a promising strategy to control insects in a species-specific manner. In this context, we review the methods for mass production of dsRNA, the approaches of exogenous application of dsRNA in the field, and the fate of dsRNA after application. Additionally, we describe the opportunities and challenges of using nanoparticles as dsRNA carriers to control insects. Furthermore, we provide future directions to improve pest management efficiency by utilizing the synergistic effects of multiple target genes. Meanwhile, the establishment of a standardized framework for assessment and regulatory consensus is critical to the commercialization of RNA pesticides.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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