Evaluation of Phenolic Content, Antioxidant Activity and Cytotoxic Effects of Ulva lactuca and Hypnea musiformis Marine Algae on MDA-MB-468 Cell Line

Abstract

Background & Objective: Breast cancer is one of the leading causes of death among women around the world. Attempts to find alternative therapies continue due to therapeutic resistance, side effects, and the high cost of conventional treatments. Materials & Methods: Hydro-methanolic extract of Ulva lactuca and Hypnea musiformis marine algae was prepared by maceration method. The phenolic content of these extracts was compared using the standard Folin-Ciocateu method and the antioxidant capacity was evaluated by the FRAP method. The cytotoxic effects of algae were compared using MTT assay and morphological changes were evaluated by an inverted microscope. Results: The phenolic content of Hypnea musiformis extract (12.46 ± 1.19 µg GAE/mg, P1000 µg/ml after 72h incubation. Conclusions: Hypnea musiformis had higher cytotoxic effects than Ulva lactuca on MDA-MB-468 cells, probably due to its higher phenolic content and antioxidant capacity. Therefore, it appears that a Hypnea musiformis alga is a better option to continue research on drug discovery of anticancer compounds.

Keywords

• algae (ulva lactuca
• hypnea musiformis)
• antioxidant
• cytotoxic effects
• breast cancer