Autoreceptor Control of Peptide/Neurotransmitter Corelease from PDF Neurons Determines Allocation of Circadian Activity in Drosophila

Charles Choi; Guan Cao; Anne K. Tanenhaus; Ellena v. McCarthy; Misun Jung; William Schleyer; Yuhua Shang; Michael Rosbash; Jerry C.P. Yin; Michael N. Nitabach

doi:10.1016/j.celrep.2012.06.021

Cell Reports (Aug 2012)

Autoreceptor Control of Peptide/Neurotransmitter Corelease from PDF Neurons Determines Allocation of Circadian Activity in Drosophila

Charles Choi,
Guan Cao,
Anne K. Tanenhaus,
Ellena v. McCarthy,
Misun Jung,
William Schleyer,
Yuhua Shang,
Michael Rosbash,
Jerry C.P. Yin,
Michael N. Nitabach

Affiliations

Charles Choi: Department of Cellular and Molecular Physiology, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
Guan Cao: Department of Cellular and Molecular Physiology, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
Anne K. Tanenhaus: Departments of Genetics and Neurology, University of Wisconsin, Madison, 3434 Genetics/Biotech, 425 Henry Mall, Madison, WI 52706, USA
Ellena v. McCarthy: Department of Cellular and Molecular Physiology, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
Misun Jung: Department of Cellular and Molecular Physiology, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
William Schleyer: Department of Cellular and Molecular Physiology, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
Yuhua Shang: Howard Hughes Medical Institute and National Center for Behavioral Genomics, Department of Biology, Brandeis University, Waltham, MA 02454, USA
Michael Rosbash: Howard Hughes Medical Institute and National Center for Behavioral Genomics, Department of Biology, Brandeis University, Waltham, MA 02454, USA
Jerry C.P. Yin: Departments of Genetics and Neurology, University of Wisconsin, Madison, 3434 Genetics/Biotech, 425 Henry Mall, Madison, WI 52706, USA
Michael N. Nitabach: Department of Cellular and Molecular Physiology, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA

DOI: https://doi.org/10.1016/j.celrep.2012.06.021
Journal volume & issue: Vol. 2, no. 2
pp. 332 – 344

Abstract

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Drosophila melanogaster flies concentrate behavioral activity around dawn and dusk. This organization of daily activity is controlled by central circadian clock neurons, including the lateral-ventral pacemaker neurons (LNvs) that secrete the neuropeptide PDF (pigment dispersing factor). Previous studies have demonstrated the requirement for PDF signaling to PDF receptor (PDFR)-expressing dorsal clock neurons in organizing circadian activity. Although LNvs also express functional PDFR, the role of these autoreceptors has remained enigmatic. Here, we show that (1) PDFR activation in LNvs shifts the balance of circadian activity from evening to morning, similar to behavioral responses to summer-like environmental conditions, and (2) this shift is mediated by stimulation of the Gα,s-cAMP pathway and a consequent change in PDF/neurotransmitter corelease from the LNvs. These results suggest another mechanism for environmental control of the allocation of circadian activity and provide new general insight into the role of neuropeptide autoreceptors in behavioral control circuits.

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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