PLoS ONE (Jan 2012)

ZEB2 mediates multiple pathways regulating cell proliferation, migration, invasion, and apoptosis in glioma.

  • Songtao Qi,
  • Ye Song,
  • Yuping Peng,
  • Hao Wang,
  • Hao Long,
  • Xiaoli Yu,
  • Zhiyong Li,
  • Luxiong Fang,
  • Aibing Wu,
  • Weiren Luo,
  • Yan Zhen,
  • Ying Zhou,
  • Yan Chen,
  • Chunping Mai,
  • Zhen Liu,
  • Weiyi Fang

DOI
https://doi.org/10.1371/journal.pone.0038842
Journal volume & issue
Vol. 7, no. 6
p. e38842

Abstract

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BackgroundThe aim of the present study was to analyze the expression of Zinc finger E-box Binding homeobox 2 (ZEB2) in glioma and to explore the molecular mechanisms of ZEB2 that regulate cell proliferation, migration, invasion, and apoptosis.Methodology/principal findingsExpression of ZEB2 in 90 clinicopathologically characterized glioma patients was analyzed by immunohistochemistry. Furthermore, siRNA targeting ZEB2 was transfected into U251 and U87 glioma cell lines in vitro and proliferation, migration, invasion, and apoptosis were examined separately by MTT assay, Transwell chamber assay, flow cytometry, and western blot.ResultsThe expression level of ZEB2 protein was significantly increased in glioma tissues compared to normal brain tissues (PConclusionOverexpression of ZEB2 is an unfavorable factor that may facilitate glioma progression. Knockdown ZEB2 expression by siRNA suppressed cell proliferation, migration, invasion and promoted cell apoptosis in glioma cells.