Effects of Moderate Exercise Training and Resveratrol Supplementation on Macrophage Infiltration and Inflammation in Adipose Tissue of Obese Mice

Abstract

PURPOSE The aim of this study was to compare the effectiveness either resveratrol or exercise with a low fat diet on visceral and subcutaneous adipose tissue of high fat diet-induced obese mice. METHODS C57BL/6 mice were fed a high fat diet for 12 weeks first and a low fat diet for 8 weeks later. Mice were divided into 5 groups: high-fat diet (HD), low-fat diet control (HLC), low-fat diet with resveratrol (HLR), low-fat diet with exercise (HLE), low-fat diet with exercise plus resveratrol (HLER). The training groups ran on a treadmill at 12-20 m/min for 30-60 min/day, 5 times/weeks for 8 week. After the 20 weeks, visceral and subcutaneous adipose tissues were analyzed. RESULTS As a result, F480 mRNA expression of HLR, HLE, and HLER was significantly decreased compared to HD in both adipose tissues. CD11c mRNA expression was significantly decreased HLE, HLER in both adipose tissue. CD163 mRNA expression was significantly increased by HLE, HLER in visceral adipose tissue only. Exercise groups were markedly inhibited TLR4 mRNA expression, ICAM-1, VCAM-1 and IL-6, TNF-α, MCP-1 mRNA in visceral adipose tissue only. In visceral and subcutaneous adipose tissue, IL-6, TNF-α mRNA were partially prevented. CONCLUSIONS These results suggest that exercise or resveratrol with a low-fat diet have a positive effect to ameliorate the obeseinduced metabolic disturbances compared to a low-fat diet alone. However, it seems that exercise with a low fat diet is more effectively macrophage infiltration in visceral than subcutaneous adipose tissue.

Keywords

• obesity
• exercise
• resveratrol
• adipose tissue
• macrophage