Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast

Sergio A. Rincon; Adam Lamson; Robert Blackwell; Viktoriya Syrovatkina; Vincent Fraisier; Anne Paoletti; Meredith D. Betterton; Phong T. Tran

doi:10.1038/ncomms15286

Nature Communications (May 2017)

Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast

Sergio A. Rincon,
Adam Lamson,
Robert Blackwell,
Viktoriya Syrovatkina,
Vincent Fraisier,
Anne Paoletti,
Meredith D. Betterton,
Phong T. Tran

Affiliations

Sergio A. Rincon: Institut Curie, PSL Research University, CNRS, UMR 144
Adam Lamson: Department of Physics, University of Colorado
Robert Blackwell: Department of Physics, University of Colorado
Viktoriya Syrovatkina: Department of Cell & Developmental Biology, University of Pennsylvania
Vincent Fraisier: Institut Curie, PSL Research University, CNRS, UMR 144
Anne Paoletti: Institut Curie, PSL Research University, CNRS, UMR 144
Meredith D. Betterton: Department of Physics, University of Colorado
Phong T. Tran: Institut Curie, PSL Research University, CNRS, UMR 144

DOI: https://doi.org/10.1038/ncomms15286
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 12

Abstract

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Bipolar spindle assembly requires a balance of kinesin 14 pulling and kinesin 5 pushing forces. Here, the authors show that in fission yeast, spindle formation can occur in the absence of kinesin 5 (Cut7) and 14 (Pkl1) but requires the microtubule-associated protein Ase1 for spindle bipolarity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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