Бюллетень сибирской медицины (Jun 2016)
INVESTIGATION OF DIURETIC ACTIVITY OF PHYTOPREPARATIONS OF CALENDULA OFFICINALIS L. FLOWERS
Abstract
The flowers of the pot marigold (Calendula officinalis L.) are part of combination herbal medicinal preparations which applied in the treatment of diseases of the hepatobiliary system, gastrointestinal tract, diseases of kidneys and the urinary tract, such as «Fitonephrol» («Urological tea»). Calendula flowers make a contribution in the total therapeutic activity of «Urological tea» as a component which has anti-inflammatory and reparative properties. As is widely known that phytopharmaceuticals usually combine the variety of therapeutic activity and softness of pharmacological effects along with the absence of a significant number of side effects and contraindications which is extremely important in the treatment of chronic pathologies. The main purpose was to study diuretic activity of phytopreparations of pot marigold.Materials and methods. Investigations of discovering of diuretic action of pot marigold’s phytopreparations were carried out on white rats of both sexes weighing 200–220 g (8 sets of experiments). Each experimental group consisted of ten animals. The effect of pot marigold medicines at a dose of 50 mg/kg was carried out background the administered intragastrically at 3% aqueous load of 4 and 24 hours of the experiment. There are such drugs as a subjects to examination: calendula tincture (1 : 10) (industrial production), calendula tincture (1 : 5) and calendula liquid extract (1 : 2) of 70% alcohol, obtained at the Department of Pharmacognosy with Botany and Bases of Phytotherapy, at a dose of 50 mg/kg. Animals were housed in the vivarium on a standard diet with free access to water. The day before the experiment the rats received intragastric water load in the amount of 3% of body weight. On the day of the experiment the animals of the control group was administered water-alcohol load and experienced – intragastrically drug dose of 50 mg/kg in an identical volume of water. As a control was used 70% alcohol. Animals were placed in metabolic cages on day. Collected urine of rats were examined. Determined excretion of water. The concentration of sodium and potassium was detected by flame photometry (mark PAG-1) for 4 and 24 hours of the experiment. The concentration of creatinine was detected by colorimetric method (mark CPHC-3) for 4 and 24 hours of the experiment. The results of experiments were performed statistical on the Mann-Whitney test. Experiments were carried out with all the necessary ethical requirements.Main results. It was found that among the studied drugs on 4 hours of the experiment the liquid extract indicate diuretic properties. Tincture (1 : 5) showed expressed diuretic activity, saluretic and keratin properties. Tincture (1 : 10) had tendency to increase diuresis. Such effects decreased within the day.Conclusion. Preparations of marigold flowers in the form of tinctures (1 : 5) and the liquid extract (1 : 2) 70% alcohol at a single intragastric administration to rats at a dose of 50 μl/kg reveal a marked diuretic effect, significantly increasing the amount of urine, excretion electrolytes and creatinine relative to the control. In consideration of the fact that preparations of Calendula flowers have antiseptic and anti-inflammatory properties, the results have a great interest in terms of the complex therapy of acute and chronic kidney and urinary tract diseases.
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