The transcriptional profile of adipose-derived stromal cells (ASC) mirrors the whitening of adipose tissue with age

Ilaria Scambi; Daniele Peroni; Alice Nodari; Flavia Merigo; Donatella Benati; Federico Boschi; Silvia Mannucci; Andrea Frontini; Silvia Visonà; Andrea Sbarbati; Mauro Krampera; Mirco Galiè

European Journal of Cell Biology (Apr 2022)

The transcriptional profile of adipose-derived stromal cells (ASC) mirrors the whitening of adipose tissue with age

Ilaria Scambi,
Daniele Peroni,
Alice Nodari,
Flavia Merigo,
Donatella Benati,
Federico Boschi,
Silvia Mannucci,
Andrea Frontini,
Silvia Visonà,
Andrea Sbarbati,
Mauro Krampera,
Mirco Galiè

Affiliations

Ilaria Scambi: Department Neuroscience, Biomedicine and Movement, Sec. Anatomy and Histology, Universitity of Verona, Verona, Italy
Daniele Peroni: Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Trento, Italy
Alice Nodari: Department Neuroscience, Biomedicine and Movement, Sec. Anatomy and Histology, Universitity of Verona, Verona, Italy
Flavia Merigo: Department Neuroscience, Biomedicine and Movement, Sec. Anatomy and Histology, Universitity of Verona, Verona, Italy
Donatella Benati: Department Neuroscience, Biomedicine and Movement, Sec. Anatomy and Histology, Universitity of Verona, Verona, Italy
Federico Boschi: Department of Computer Science, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy
Silvia Mannucci: Department Neuroscience, Biomedicine and Movement, Sec. Anatomy and Histology, Universitity of Verona, Verona, Italy
Andrea Frontini: Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy
Silvia Visonà: Department of Public Health Experimental and Forensic Medicine, University of Pavia, Pavia, Italy
Andrea Sbarbati: Department Neuroscience, Biomedicine and Movement, Sec. Anatomy and Histology, Universitity of Verona, Verona, Italy
Mauro Krampera: Department of Medicine, Section Hematology, Stem Cell Research Laboratory, University of Verona, Italy
Mirco Galiè: Department Neuroscience, Biomedicine and Movement, Sec. Anatomy and Histology, Universitity of Verona, Verona, Italy; Correspondence to: Dept. Neuriscience, Biomedicine and Movement – Sec. Anatomy and HIstology, University of Verona, Via Le Grazie 8, 37134 Verona, Italy.

Journal volume & issue: Vol. 101, no. 2
p. 151206

Abstract

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Multipotent stem cells persist within the stromal vascular fraction (SVF) of adipose tissue during adulthood. These cells, commonly referred to as adipose-derived stromal cells (ASC), have been extensively investigated over the past years as a promising therapeutic tool based on their regenerative and immunomodulatory properties. However, how ASC might mirror the age-related alteration of the fat they reside in remains unclear. Herein, we show that inguinal adipose tissue in mice turns from brown/beige- to white-like with age and resident ASC readily mirror these changes both at mRNA and microRNA transcriptional level. Mechanistically, our data suggest that these brown/age-related changes in ASC transcription rely on changes in the activity of E2F1 and NFkB transcription factors.

Published in European Journal of Cell Biology

ISSN: 0171-9335 (Print); 1618-1298 (Online)
Publisher: Elsevier
Country of publisher: Germany
LCC subjects: Science: Biology (General): Cytology
Website: https://www.journals.elsevier.com/european-journal-of-cell-biology

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